Estimation of Staphylococcus total viable count in different contamination states of central venous catheters in hemodialysis centers based on tunable diode laser wavelength-modulation spectroscopy

IF 1 4区工程技术 Q4 ENGINEERING, ELECTRICAL & ELECTRONIC

Microwave and Optical Technology Letters Pub Date : 2024-10-05 DOI:10.1002/mop.34329

Xueli Wu, Qi Li, Jia Chen, Qiaoyan Chen, Meifang Yu

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Abstract

The surveillance and assessment of the total viable count (TVC) of Staphylococcus under different levels of catheter contamination are crucial for the prevention and evaluation of catheter-related infections in hemodialysis (HD). This paper demonstrates the noninvasive and accurate measurement of the TVC of Staphylococcus using wavelength-modulated tunable diode laser absorption spectroscopy (WMAS). The tunable distributed feedback laser with a central wavelength of 2004 nm was employed and the detection limit of 22.5 ppm for carbon dioxide (CO₂) produced by Staphylococcus epidermidis was obtained. The growth of Staphylococcus under different medium concentrations was studied, and the results indicated a strong correlation between the growth rate and final TVC of S. epidermidis with the medium concentration. When the degree of catheter contamination was low, resulting in minimal residual nutrient concentration within the catheter, the bacterial growth rate and TVC were effectively suppressed. Therefore, it is proven that WMAS is a user-friendly, noninvasive, and high signal-to-noise ratio technique for monitoring Staphylococcus, making it an effective tool for assessing Staphylococcus growth in HD catheter surveillance.

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基于可调谐二极管激光波长调制光谱法估算血液透析中心中心静脉导管不同污染状态下的葡萄球菌总存活数

监测和评估不同导管污染程度下葡萄球菌的总存活数（TVC）对于预防和评估血液透析（HD）中导管相关感染至关重要。本文展示了利用波长调制可调谐二极管激光吸收光谱（WMAS）对葡萄球菌的 TVC 进行无创、精确测量的方法。采用中心波长为 2004 nm 的可调谐分布式反馈激光，表皮葡萄球菌产生的二氧化碳（CO2）的检测限为 22.5 ppm。研究了不同培养基浓度下葡萄球菌的生长情况，结果表明表皮葡萄球菌的生长速度和最终 TVC 与培养基浓度密切相关。当导管污染程度较低，导致导管内残留的营养浓度极低时，细菌的生长速度和 TVC 都会被有效抑制。因此，事实证明 WMAS 是一种用户友好型、无创、高信噪比的葡萄球菌监测技术，是在高清导管监测中评估葡萄球菌生长情况的有效工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microwave and Optical Technology Letters 工程技术-工程：电子与电气

CiteScore

3.40

自引率

20.00%

发文量

371

审稿时长

4.3 months

期刊介绍： Microwave and Optical Technology Letters provides quick publication (3 to 6 month turnaround) of the most recent findings and achievements in high frequency technology, from RF to optical spectrum. The journal publishes original short papers and letters on theoretical, applied, and system results in the following areas. - RF, Microwave, and Millimeter Waves - Antennas and Propagation - Submillimeter-Wave and Infrared Technology - Optical Engineering All papers are subject to peer review before publication