Preparation of a chitosan/polyvinyl alcohol-based dual-network hydrogel for use as a potential wound-healing material for the sustainable release of drugs

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2024-10-08 DOI:10.1016/j.carbpol.2024.122822

Hongyang Guo , Haiying Luo , Juanying Ou , Jie Zheng , Caihuan Huang , Fu Liu , Shiyi Ou

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引用次数: 0

Abstract

Treating chronic wounds poses significant challenges in clinical medicine due to bacterial infection, reactive oxygen species (ROS) accumulation, and excessive inflammation. This study aimed to address these issues by developing a wound dressing with antibacterial, antioxidant, and anti-inflammatory properties. Chitosan was functionally modified with acrolein to covalently bind to epigallocatechin gallate (EGCG), enabling a high EGCG load. Subsequently, polyvinyl alcohol (PVA) and EGCG-modified chitosan were crosslinked to prepare a new double-network hydrogel with added cysteine (CSAEC/P50). CSAEC/P50 demonstrated optimal mechanical properties (low swelling rate, high water retention, and optimal flexibility), low hemolysis, high coagulation properties, and antibacterial and antioxidant activities. Cell scratch tests indicated that CSAEC/P50 can promote NIH3T3 cell migration. Immunofluorescence results showed that CSAEC/P50 promoted the transformation of proinflammatory M₁ macrophages to anti-inflammatory M₂ macrophages. These findings suggest that CSAEC/P50 has significant potential for use in wound dressing applications.

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制备壳聚糖/聚乙烯醇双网络水凝胶，将其用作可持续释放药物的潜在伤口愈合材料

由于细菌感染、活性氧（ROS）积累和过度炎症，治疗慢性伤口给临床医学带来了巨大挑战。本研究旨在通过开发一种具有抗菌、抗氧化和抗炎特性的伤口敷料来解决这些问题。用丙烯醛对壳聚糖进行了功能修饰，使其与表没食子儿茶素没食子酸酯（EGCG）共价结合，从而实现了高 EGCG 负载。随后，聚乙烯醇（PVA）与 EGCG 改性壳聚糖交联，制备出添加了半胱氨酸的新型双网水凝胶（CSAEC/P50）。CSAEC/P50 具有最佳的机械性能（低膨胀率、高保水性和最佳柔韧性）、低溶血率、高凝结性能以及抗菌和抗氧化活性。细胞划痕试验表明，CSAEC/P50 可促进 NIH3T3 细胞迁移。免疫荧光结果显示，CSAEC/P50 能促进促炎性 M1 巨噬细胞向抗炎性 M2 巨噬细胞转化。这些研究结果表明，CSAEC/P50 在伤口敷料应用方面具有巨大的潜力。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.