Development an automated and high -throughput analysis methods for detecting 64 anabolic androgenic steroids in urine using gas chromatography-mass spectrometry

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open Pub Date : 2024-10-01 DOI:10.1016/j.talo.2024.100362

Zhaobo Wu , Aoxin Ma , Lili Xing , Xiaochen Huang , Guojun Li , Kaoqi Lian

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Abstract

A gas chromatography tandem mass spectrometry (GC–MS/MS) assay was developed for the simultaneous detection of 64 anabolic androgenic steroids (AAS). Urine is extracted with tertbutyl methyl ether under alkaline conditions, purified with C18, and derived through silanization, Detection is carried out in dynamic multiple reaction monitoring (dMRM) mode, The overall recoveries ranged from 74.31 % to 119.66 %, The relative standard deviation is <10 %, with a linear range of 1–200 ng/ml, The linear range of endogenous androsterone (A) and etiocholanolone (Etio) is 1.7–10,000 ng/ml, with a correlation coefficient 0.9991– 0.9999. The limit of detection (LOD) range of this experiment was 0.2 ng/ml-1.2 ng/ml, and limit of quantification (LOQ) was 0.7 ng/ml-4 ng/ml, The results of testing 100 actual samples show that this method is simple, effective and feasible, and can be used for the detection of AAS, meeting the requirements of the World Anti Doping Agency (WADA)

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利用气相色谱-质谱法开发自动化高通量分析方法，检测尿液中的 64 种合成代谢雄性类固醇

开发了一种气相色谱串联质谱（GC-MS/MS）测定法，用于同时检测 64 种同化雄性类固醇（AAS）。尿液在碱性条件下用叔丁基甲醚提取，经 C18 纯化，硅烷化衍生，以动态多反应监测（dMRM）模式进行检测。内源性雄性酮（A）和乙酰胆碱酮（Etio）的线性范围为 1.7-10,000 ng/ml，相关系数为 0.9991- 0.9999。本实验的检出限（LOD）范围为 0.2 纳克/毫升-1.2 纳克/毫升，定量限（LOQ）为 0.7 纳克/毫升-4 纳克/毫升，100 份实际样品的检测结果表明该方法简单、有效、可行，可用于 AAS 的检测，符合世界反兴奋剂机构（WADA）的要求。

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