Genome-wide identification and expression analysis of SlKFB gene family (Solanum lycopersicum) and the molecular mechanism of SlKFB16 and SlKFB34 under drought

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-10-10 DOI:10.1016/j.plaphy.2024.109192

Lei Yu , Xiaoyu Guan , Fanyue Meng , Fulei Mo , Rui Lv , Zhen Ding , Peiwen Wang , Xiuling Chen , Mozhen Cheng , Aoxue Wang

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Abstract

Environmental stress significantly affects plant growth and productivity. The effects of drought stress on plants are reflected primarily in enzyme activity, membrane systems, and cell-water loss. Here, the Kelch repeat F-box (KFB) protein family in tomato was systematically identified and analysed. Using bioinformatics, we identified 37 SlKFB family members in the tomato genome and analysed their protein structure, phylogenetic relationships, chromosome distribution, and expression under drought or biotic-stress conditions. Transcriptome data revealed that SlKFB members exhibit differential responses to drought stress, with significant differences in SlKFB16 and SlKFB34 expression. Functional analysis revealed that SlKFB16 functions in the cytoplasm and SlKFB34 in the nucleus and cytoplasm. Under drought stress, SlKFB16 and SlKFB34-silencing significantly reduced reactive oxygen species scavenging and resistance to drought stress. These findings provide a reference for further studies of the mechanisms of SlKFB16 and SlKFB34 in drought stress in tomato as well as a foundation for enhancing their resistance to drought stress.

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SlKFB基因家族（Solanum lycopersicum）的全基因组鉴定和表达分析以及SlKFB16和SlKFB34在干旱条件下的分子机制

环境胁迫会严重影响植物的生长和生产力。干旱胁迫对植物的影响主要体现在酶活性、膜系统和细胞失水等方面。在此，我们对番茄中的 Kelch 重复 F-box （KFB）蛋白家族进行了系统鉴定和分析。利用生物信息学方法，我们鉴定了番茄基因组中的 37 个 SlKFB 家族成员，并分析了它们的蛋白质结构、系统发育关系、染色体分布以及在干旱或生物胁迫条件下的表达情况。转录组数据显示，SlKFB家族成员对干旱胁迫表现出不同的反应，其中SlKFB16和SlKFB34的表达存在显著差异。功能分析显示，SlKFB16在细胞质中发挥作用，SlKFB34在细胞核和细胞质中发挥作用。在干旱胁迫下，SlKFB16和SlKFB34的沉默会显著降低活性氧清除能力和对干旱胁迫的抗性。这些发现为进一步研究SlKFB16和SlKFB34在番茄干旱胁迫中的作用机制提供了参考，也为增强其对干旱胁迫的抗性奠定了基础。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.