Long lasting degradation of all alkanes in soil by Pseudomonas activated after Fenton pre-oxidation

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2024-10-02 DOI:10.1016/j.bej.2024.109511

Jinlan Xu , Yikai Li , Mengzhen Gao , Jianan Dai , Huan Li , Jiayi Wang

{"title":"Long lasting degradation of all alkanes in soil by Pseudomonas activated after Fenton pre-oxidation","authors":"Jinlan Xu , Yikai Li , Mengzhen Gao , Jianan Dai , Huan Li , Jiayi Wang","doi":"10.1016/j.bej.2024.109511","DOIUrl":null,"url":null,"abstract":"<div><div>This study investigated the function and mechanism of Fenton pre-oxidation on the long lasting degradation of all alkanes in soil contaminated by petroleum. The findings demonstrated that the biological removal amount of all alkanes in the respiratory regulation group reached 4083.46 mg/kg, which was twice that of the non-regulation group, and the removal amount gradually increased in the four stages of bioremediation. In addition, the removal amount of all alkanes in the non-regulated group did not change much and showed a downward trend, indicating that long lasting degradation of all alkanes could be achieved by the respiratory regulation group, and the biodegradation cycle was saved by 251 days compared with the non-regulated group. Furthermore, the total number of bacteria in the respiratory regulation group (6.73 log CFU/g) was significantly higher than that in the non-regulation group (2.25 log CFU/g). <em>Pseudomonas</em> became the dominant genus in the respiratory regulation group with an average relative abundance of 32.17 %. In the respiratory regulation group, a large amount of ammonia nitrogen (1703.62 mg/kg) was consumed during the degradation process, which stimulated the tricarboxylic acid cycle respiratory metabolism process of <em>Pseudomonas</em> and accelerated the hydrocarbon conversion. This may be the reason why the long lasting degradation of all alkanes in soil could be achieved by the respiratory regulation group.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"212 ","pages":"Article 109511"},"PeriodicalIF":3.7000,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369703X24002985","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

This study investigated the function and mechanism of Fenton pre-oxidation on the long lasting degradation of all alkanes in soil contaminated by petroleum. The findings demonstrated that the biological removal amount of all alkanes in the respiratory regulation group reached 4083.46 mg/kg, which was twice that of the non-regulation group, and the removal amount gradually increased in the four stages of bioremediation. In addition, the removal amount of all alkanes in the non-regulated group did not change much and showed a downward trend, indicating that long lasting degradation of all alkanes could be achieved by the respiratory regulation group, and the biodegradation cycle was saved by 251 days compared with the non-regulated group. Furthermore, the total number of bacteria in the respiratory regulation group (6.73 log CFU/g) was significantly higher than that in the non-regulation group (2.25 log CFU/g). Pseudomonas became the dominant genus in the respiratory regulation group with an average relative abundance of 32.17 %. In the respiratory regulation group, a large amount of ammonia nitrogen (1703.62 mg/kg) was consumed during the degradation process, which stimulated the tricarboxylic acid cycle respiratory metabolism process of Pseudomonas and accelerated the hydrocarbon conversion. This may be the reason why the long lasting degradation of all alkanes in soil could be achieved by the respiratory regulation group.

查看原文本刊更多论文

经 Fenton 预氧化处理的假单胞菌对土壤中所有烷烃的长效降解作用

本研究探讨了 Fenton 预氧化作用对石油污染土壤中所有烷烃的长效降解作用及其机理。研究结果表明，呼吸调节组对所有烷烃的生物去除量达到 4083.46 mg/kg，是非调节组的两倍，并且在生物修复的四个阶段中去除量逐渐增加。此外，非调节组对所有烷烃的去除量变化不大，呈下降趋势，这表明呼吸调节组可实现对所有烷烃的长效降解，生物降解周期比非调节组节省了 251 天。此外，呼吸调节组的细菌总数（6.73 log CFU/g）明显高于非调节组（2.25 log CFU/g）。假单胞菌成为呼吸调节组的优势菌属，平均相对丰度为 32.17%。呼吸调节组在降解过程中消耗了大量氨氮（1703.62 mg/kg），刺激了假单胞菌的三羧酸循环呼吸代谢过程，加速了碳氢化合物的转化。这可能就是呼吸调节组能够长期降解土壤中所有烷烃的原因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.