Vermicomposting reduces the antimicrobial resistance in livestock waste

IF 5.4 Q2 ENGINEERING, ENVIRONMENTAL
Masaru Usui , Akira Fukuda , Takashi Azuma , Yoshihiro Kobae , Yuichi Hori , Mitsutaka Kushima , Satoshi Katada , Chie Nakajima , Yasuhiko Suzuki
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Abstract

Vermicomposting, a process in which housefly larvae are used to decompose organic waste, has attracted attention as a method for managing antimicrobial-resistant bacteria (ARB) in livestock manure. Vermicomposting effectively reduces antimicrobial resistance genes (ARGs) and residual antimicrobials. However, the evaluation of live bacteria, including ARB, remains scarce. Additionally, conventional DNA extraction methods include DNA from dead bacteria, impeding the accurate evaluation of ARG-associated risk in compost and the microbiome. This study assesses the effectiveness of vermicomposting pig manure against antimicrobial resistance (AMR) by evaluating the ARB, ARGs (focusing on DNA from live bacteria), and microbiome associated with vermicomposting processes. Vermicomposting significantly reduces the abundance of bacteria, including ARB, and decreases the ARG (tetA, tetB, blaTEM, and blaCTX−M) copy number in live bacteria. Bacterial community analysis revealed an increase in the abundance of Gammaproteobacteria. Moreover, the vermicomposted samples effectively cultivated myriad plants. Overall, vermicomposting effectively reduces the ARB and ARGs in pig manure, with potential benefits for plant growth and sustainable waste management. Hence, it can be widely applied to treat livestock manure and other organic wastes to combat AMR.

Abstract Image

蚯蚓堆肥可减少牲畜粪便中的抗微生物抗药性
蚯蚓堆肥是一种利用家蝇幼虫分解有机废物的方法,它作为一种管理牲畜粪便中抗菌素耐药菌(ARB)的方法而备受关注。蚯蚓堆肥可有效减少抗菌药耐药性基因(ARGs)和残留抗菌药。然而,对活细菌(包括 ARB)的评估仍然很少。此外,传统的 DNA 提取方法包括死细菌的 DNA,妨碍了对堆肥和微生物组中与 ARG 相关的风险进行准确评估。本研究通过评估与蚯蚓堆肥过程相关的 ARB、ARGs(侧重于活细菌的 DNA)和微生物组,评估了猪粪蚯蚓堆肥对抗菌剂耐药性(AMR)的有效性。蚯蚓堆肥大大降低了细菌(包括 ARB)的丰度,并减少了活细菌中 ARG(tetA、tetB、blaTEM 和 blaCTX-M)的拷贝数。细菌群落分析表明,伽马蛋白菌的数量有所增加。此外,蚯蚓堆肥样本还能有效栽培多种植物。总之,蚯蚓堆肥可有效减少猪粪中的 ARB 和 ARGs,对植物生长和可持续废物管理具有潜在益处。因此,蚯蚓堆肥可广泛应用于处理牲畜粪便和其他有机废物,以防治 AMR。
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来源期刊
Journal of hazardous materials advances
Journal of hazardous materials advances Environmental Engineering
CiteScore
4.80
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