Citrus exosome-modified exogenous dsRNA delivery reduces plant pathogen resistance and mycotoxin production

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology Pub Date : 2024-09-28 DOI:10.1016/j.pestbp.2024.106151

Chunxiao Yin , Yuli Lao , Lihong Xie , Lianfei Chen , Yueming Jiang , Liang Gong

{"title":"Citrus exosome-modified exogenous dsRNA delivery reduces plant pathogen resistance and mycotoxin production","authors":"Chunxiao Yin , Yuli Lao , Lihong Xie , Lianfei Chen , Yueming Jiang , Liang Gong","doi":"10.1016/j.pestbp.2024.106151","DOIUrl":null,"url":null,"abstract":"<div><div>Plant-derived exosome-like nanoparticles (PENs) are crucial for intercellular communication. However, PEN-based transport of pathogenic fungal genes remains unclear. This study isolated and purified PENs from lane late navel orange citrus juice by following the sucrose gradient ultracentrifugation technique. Citrus PENs were round and oval-shaped with an average size of 154.5 ± 1.9 nm. Electroporation-based exogenous dsRNA to PENs loading efficiency remained at 6.0 %. Laser confocal microscopy was employed to investigate citrus PEN uptake by fungal spores. dsCrcB loaded PENs inhibited the CrcB gene expression in spores to alleviate <em>Penicillium italicum</em> resistance against prochloraz fungicide, which promoted resistant strains' mortality by 10-fold. Moreover, dsFUM21-loaded PENs suppressed the FUM21 gene expression in spores, which significantly reduced FB1 production in <em>Fusarium proliferatum</em>. These findings suggest that citrus PENs could potentially serve as nano-carriers to counter fungicide resistance and mycotoxin production in pathogenic plant fungi.</div></div>","PeriodicalId":19828,"journal":{"name":"Pesticide Biochemistry and Physiology","volume":"205 ","pages":"Article 106151"},"PeriodicalIF":4.2000,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesticide Biochemistry and Physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0048357524003845","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Plant-derived exosome-like nanoparticles (PENs) are crucial for intercellular communication. However, PEN-based transport of pathogenic fungal genes remains unclear. This study isolated and purified PENs from lane late navel orange citrus juice by following the sucrose gradient ultracentrifugation technique. Citrus PENs were round and oval-shaped with an average size of 154.5 ± 1.9 nm. Electroporation-based exogenous dsRNA to PENs loading efficiency remained at 6.0 %. Laser confocal microscopy was employed to investigate citrus PEN uptake by fungal spores. dsCrcB loaded PENs inhibited the CrcB gene expression in spores to alleviate Penicillium italicum resistance against prochloraz fungicide, which promoted resistant strains' mortality by 10-fold. Moreover, dsFUM21-loaded PENs suppressed the FUM21 gene expression in spores, which significantly reduced FB1 production in Fusarium proliferatum. These findings suggest that citrus PENs could potentially serve as nano-carriers to counter fungicide resistance and mycotoxin production in pathogenic plant fungi.

Abstract Image

查看原文本刊更多论文

柑橘外泌体修饰的外源 dsRNA 递送可降低植物病原体抗性和霉菌毒素产量

源自植物的类外泌体纳米颗粒（PENs）对细胞间通信至关重要。然而，基于 PEN 的病原真菌基因运输仍不清楚。本研究采用蔗糖梯度超速离心技术，从晚熟脐橙柑橘汁中分离纯化了PENs。柑橘 PEN 呈圆形和椭圆形，平均大小为 154.5 ± 1.9 nm。电穿孔法将外源 dsRNA 加载到 PENs 的效率保持在 6.0%。dsCrcB负载的PENs抑制了孢子中CrcB基因的表达，从而减轻了意大利青霉对丙草胺杀菌剂的抗性，使抗性菌株的死亡率提高了10倍。此外，负载 dsFUM21 的五胜肽能抑制孢子中 FUM21 基因的表达，从而显著降低增殖镰刀菌的 FB1 产量。这些研究结果表明，柑橘五胜肽有可能作为纳米载体来对抗植物病原真菌对杀菌剂的抗性和霉菌毒素的产生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.

文献相关原料

公司名称	产品信息	采购帮参考价格
上海源叶	Fumonisin standards
上海源叶	other compounds