Utilizing Mitracarpus scaber extracts for green synthesis of silver nanoparticles: Exploring physicochemical properties and potential chemopreventive activity against N-methyl-nitrosourea- induced prostate carcinoma in rats

IF 5.45 Q1 Physics and Astronomy

Nano-Structures & Nano-Objects Pub Date : 2024-10-01 DOI:10.1016/j.nanoso.2024.101363

Temitayo I. Adesipe , Emeka J. Iweala , Ismail O. Ishola , Omotayo A. Arotiba , Abiodun H. Adebayo

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引用次数: 0

Abstract

This study evaluated the influence of silver nanoparticles (AgNPs) biosynthesized using Mitracarpus scaber (M. scaber) extracts on testosterone and n-methyl-nitrosourea (MNU)-induced prostate carcinoma in rat. AgNPs were synthesized from 0.1 M AgNO₃ solution using the aqueous and ethanol extracts of M. scaber (AMS and EMS) as reducing as well as capping agents. The AgNPs produced using AMS (ANP) and EMS (ENP) were then analyzed via various spectroscopic experiments. Later on, the biological effects of ANP and ENP were evaluated on testosterone and n-methyl-nitrosourea (MNU)-induced prostate carcinoma in rat. The study found that ANP and ENP have characteristic crystalline structures, with particle sizes ranging from ∼5–20 nm and prominent absorbance peak at 425 nm was observed for ANP while absorption peaks at 410 and 675 nm were observed for ENP indicating that ANP is isotropic in nature while ENP is anisotropic in nature. The findings regarding chemopreventive effects on prostate carcinogenesis revealed that ANP caused a significant (p < 0.05) reduction in prostate weight. However, both ANP and ENP, ameliorated prostatic hypertrophy (i.e., decreased prostate enlargement and acini proliferation) in rats induced with high-grade prostatic intraepithelial neoplasia. In comparison to the PCa group as well as other groups, ENP significantly (p < 0.05) decreased the mean concentrations of prostate-specific antigen (PSA), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-). Also, ENP significantly restored depleted activities levels of superoxide dismutase (SOD) and catalase caused by prostate carcinogenesis. Furthermore, ENP caused a significant (p < 0.05) reduction in glutathione (GSH) levels, an upregulation of DNA methyltransferase (DNMT1 and DNMT3b) expression, and activation of caspase 7. The results of the present study showed the potential anti-inflammatory, antioxidant and anti-neoplastic effects of AgNPs of M. scaber ethanol extract which implies that it could be used as an adjunct in the treatment of prostate cancer.

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利用糙米提取物绿色合成银纳米粒子：探索N-甲基亚硝基脲诱导的大鼠前列腺癌的理化性质和潜在化学预防活性

本研究评估了利用疮痂树（Mitracarpus scaber，M. scaber）提取物生物合成的银纳米粒子（AgNPs）对睾酮和亚硝基脲（n-methyl-nitrosourea，MNU）诱导的大鼠前列腺癌的影响。以葶苈子的水提取物和乙醇提取物（AMS和EMS）为还原剂和封端剂，从0.1 M AgNO3溶液中合成了AgNPs。使用 AMS（ANP）和 EMS（ENP）制备的 AgNPs 随后通过各种光谱实验进行了分析。随后，研究人员评估了 ANP 和 ENP 对睾酮和 n-甲基亚硝基脲（MNU）诱导的大鼠前列腺癌的生物效应。研究发现，ANP 和 ENP 具有特征性的结晶结构，粒径范围为 5-20 纳米，ANP 在 425 纳米波长处有明显的吸收峰，而 ENP 在 410 纳米波长和 675 纳米波长处有吸收峰，这表明 ANP 具有各向同性，而 ENP 具有各向异性。关于对前列腺癌发生的化学预防作用的研究结果表明，ANP 能显著（p < 0.05）减少前列腺重量。不过，ANP 和 ENP 都能改善高级别前列腺上皮内瘤变大鼠的前列腺肥大（即减少前列腺增大和尖头增生）。与 PCa 组和其他组相比，ENP 能显著降低前列腺特异性抗原（PSA）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-）的平均浓度（p < 0.05）。此外，ENP 还能明显恢复前列腺癌导致的超氧化物歧化酶（SOD）和过氧化氢酶的活性水平。此外，ENP 还会导致谷胱甘肽（GSH）水平明显降低（p < 0.05）、DNA 甲基转移酶（DNMT1 和 DNMT3b）表达上调以及 caspase 7 激活。本研究结果表明，疥螨乙醇提取物中的 AgNPs 具有潜在的抗炎、抗氧化和抗肿瘤作用，这意味着它可用作治疗前列腺癌的辅助药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano-Structures & Nano-Objects Physics and Astronomy-Condensed Matter Physics

CiteScore

9.20

自引率

0.00%

发文量

审稿时长

22 days

期刊介绍： Nano-Structures & Nano-Objects is a new journal devoted to all aspects of the synthesis and the properties of this new flourishing domain. The journal is devoted to novel architectures at the nano-level with an emphasis on new synthesis and characterization methods. The journal is focused on the objects rather than on their applications. However, the research for new applications of original nano-structures & nano-objects in various fields such as nano-electronics, energy conversion, catalysis, drug delivery and nano-medicine is also welcome. The scope of Nano-Structures & Nano-Objects involves: -Metal and alloy nanoparticles with complex nanostructures such as shape control, core-shell and dumbells -Oxide nanoparticles and nanostructures, with complex oxide/metal, oxide/surface and oxide /organic interfaces -Inorganic semi-conducting nanoparticles (quantum dots) with an emphasis on new phases, structures, shapes and complexity -Nanostructures involving molecular inorganic species such as nanoparticles of coordination compounds, molecular magnets, spin transition nanoparticles etc. or organic nano-objects, in particular for molecular electronics -Nanostructured materials such as nano-MOFs and nano-zeolites -Hetero-junctions between molecules and nano-objects, between different nano-objects & nanostructures or between nano-objects & nanostructures and surfaces -Methods of characterization specific of the nano size or adapted for the nano size such as X-ray and neutron scattering, light scattering, NMR, Raman, Plasmonics, near field microscopies, various TEM and SEM techniques, magnetic studies, etc .