N6-methyladenosine-modified lncRNA in Staphylococcus aureus-injured bovine mammary epithelial cells

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Haojun Xu, Xuan Wu, Zhiming Yang, Xinhuai Shi, Aizhen Guo, Changmin Hu
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引用次数: 0

Abstract

Staphylococcus aureus-induced mastitis is a serious disease in dairy bovine, with no currently effective treatment. Antibiotics demonstrate certain therapeutic potency in dairy husbandry; they generate drug-resistant bacteria, thereby harming public health. LncRNAs and m6A have been verified as potential targets in infectious diseases and have powerful regulatory capabilities. However, the biological regulation of lncRNAs with m6A modification in mastitis needs further investigation. This study aims to determine the m6A-modified lncRNAs in bovine mammary epithelial cells and their diversity during S. aureus induction. Heat-inactivated S. aureus was used to develop the cell injury model, and we subsequently found low cell viability and different m6A modification levels. Our analysis of m6A-modified lncRNA profiles through MeRIP-seq revealed significant differences in 140 peaks within 130 lncRNAs when cells were injured by S. aureus. Furthermore, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that these differential m6A-modified lncRNAs were mainly enriched in the WNT pathway, and their functions were associated with amino acid metabolism, lipid translocation, and metalloproteinase activity. Here, we report for the first time lncRNAs with m6A modification in regulating S. aureus infection, revealing potential mechanisms and targets of infectious diseases, such as mastitis, from an epigenetics perspective.

金黄色葡萄球菌损伤的牛乳腺上皮细胞中的 N6-甲基腺苷修饰 lncRNA
金黄色葡萄球菌引起的乳腺炎是奶牛的一种严重疾病,目前尚无有效的治疗方法。抗生素在奶牛饲养中显示出一定的治疗效力,但它们会产生耐药细菌,从而危害公众健康。LncRNA 和 m6A 已被证实是传染性疾病的潜在靶标,具有强大的调控能力。然而,lncRNAs与m6A修饰在乳腺炎中的生物学调控还需要进一步研究。本研究旨在确定牛乳腺上皮细胞中经m6A修饰的lncRNA及其在金黄色葡萄球菌诱导过程中的多样性。我们使用热灭活的金黄色葡萄球菌建立细胞损伤模型,随后发现细胞存活率低且m6A修饰水平不同。我们通过MeRIP-seq分析了m6A修饰的lncRNA图谱,发现当细胞受到金黄色葡萄球菌损伤时,130个lncRNA中的140个峰存在显著差异。此外,基因本体(Gene Ontology)和京都基因组百科全书(Kyoto Encyclopedia of Genes and Genomes)富集分析表明,这些差异的m6A修饰lncRNA主要富集于WNT通路,其功能与氨基酸代谢、脂质转运和金属蛋白酶活性有关。在此,我们首次报道了具有m6A修饰的lncRNA在调控金黄色葡萄球菌感染中的作用,从表观遗传学的角度揭示了乳腺炎等感染性疾病的潜在机制和靶标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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