The applicability of the SERS technique in food contamination testing – The detailed spectroscopic, chemometric, genetic, and comparative analysis of food-borne Cronobacter spp. strains

IF 5 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

International journal of food microbiology Pub Date : 2024-10-03 DOI:10.1016/j.ijfoodmicro.2024.110930

K. Niciński , E. Witkowska , D. Korsak , M. Szuplewska , A. Kamińska

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引用次数: 0

Abstract

Microorganisms assigned as Cronobacter are Gram-negative, facultatively anaerobic, bacteria widely distributed in nature, home environments, and hospitals. They can also be detected in foods, milk powder, and powdered infant formula (PIF). Additionally, as an opportunistic pathogen, Cronobacter may cause serious infections, sometimes leading to the death of neonates and infants. Thus, it is essential to test food products for the presence of Cronobacter spp. The currently used standard described in ISO 22964:2017 is a laborious method that could be easily replaced by surface-enhanced Raman scattering (SERS).

Here, we demonstrate that SERS allows the identification of food-borne bacteria belonging to Cronobacter spp. based on their SERS spectra. For this purpose, twenty-six Cronobacter strains from different food samples were analyzed. Additionally, it was shown that it is possible to differentiate them from other closely related pathogens such as Salmonella enterica subsp. enterica, Escherichia coli, or Enterobacter spp. The SERS results were supported by principal component analysis (PCA), as well as and sequencing of 16S rRNA, rpoB and fusA genes. Last but not least, it was demonstrated that the cells of Cronobacter sakazakii may be easily separated from PIF using an appropriate filter, microfluidic chip, and dielectrophoresis (DEP) technique.

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SERS 技术在食品污染检测中的适用性 - 对食源性克罗诺杆菌属菌株进行详细的光谱、化学计量、遗传和比较分析。

克罗诺杆菌是一种革兰氏阴性、兼性厌氧细菌，广泛分布于自然界、家庭环境和医院中。在食品、奶粉和婴儿配方粉（PIF）中也能检测到它们。此外，作为一种机会性病原体，克罗诺杆菌可能会引起严重感染，有时会导致新生儿和婴儿死亡。ISO 22964:2017 中描述的当前使用的标准是一种费力的方法，很容易被表面增强拉曼散射（SERS）所取代。在这里，我们证明了 SERS 可以根据其 SERS 光谱来识别属于克罗诺杆菌属的食源性细菌。为此，我们分析了来自不同食品样本的 26 株克罗诺杆菌。主成分分析（PCA）以及 16S rRNA、rpoB 和 fusA 基因的测序也支持了 SERS 的结果。最后但并非最不重要的一点是，该研究证明，使用适当的过滤器、微流控芯片和介电泳（DEP）技术，可以很容易地从 PIF 中分离出阪崎肠杆菌的细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International journal of food microbiology 工程技术-食品科技

CiteScore

10.40

自引率

5.60%

发文量

322

审稿时长

65 days

期刊介绍： The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.