UBE2Q1 as a novel cancer biomarker for lung adenocarcinoma: Short Title: Oncogenic function of UBE2Q1 in lung adenocarcinoma.

Wei Jiang, Yuchao Wang, Jue Zou, Li Li, Chunhua Xu
{"title":"UBE2Q1 as a novel cancer biomarker for lung adenocarcinoma: Short Title: Oncogenic function of UBE2Q1 in lung adenocarcinoma.","authors":"Wei Jiang, Yuchao Wang, Jue Zou, Li Li, Chunhua Xu","doi":"10.1016/j.amjms.2024.10.002","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Ubiquitin-conjugating enzymes (E2s) participate in various tumor-promoting processes. UBE2Q1 is a member of the E2 family. This research aimed to detect the expression level of UBE2Q1 in human lung adenocarcinoma and to study its malignant biological function.</p><p><strong>Methods: </strong>Western blot, qRT-PCR and immunohistochemistry was used to measure the expression of UBE2Q1 in human lung adenocarcinoma tissues. The association between UBE2Q1 expression and clinic-pathological variables in 99 lung adenocarcinoma samples was analyzed by immunohistochemistry. In vitro experiment, establishing UBE2Q1 knockdown pattern, the markers of apoptosis, cell cycle and epithelial-mesenchymal transition (EMT) were analyzed by Western blot. CCK8, colony formation, Transwell and invasion assay analyzed the effect of UBE2Q1 knockdown on the proliferation, metastasis and invasion of lung cancer cells.</p><p><strong>Results: </strong>UBE2Q1 was overexpressed in lung adenocarcinoma, and the expression level of UBE2Q1 was related with TNM stage, tumor size, and lymph node metastasis. The high level of UBE2Q1 expression was also associated with poor survival and was an independent risk factor. In vitro, It was also confirmed that steady downregulation of UBE2Q1 could promote apoptosis, induce G2/M cell cycle arrest and regulate EMT. UBE2Q1 silencing dramatically reduce lung tumor cells proliferation, migration and invasion capacities.</p><p><strong>Conclusions: </strong>UBE2Q1 may serve as a prognostic biomarker and a new therapeutic target of lung adenocarcinoma.</p>","PeriodicalId":94223,"journal":{"name":"The American journal of the medical sciences","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The American journal of the medical sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.amjms.2024.10.002","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Purpose: Ubiquitin-conjugating enzymes (E2s) participate in various tumor-promoting processes. UBE2Q1 is a member of the E2 family. This research aimed to detect the expression level of UBE2Q1 in human lung adenocarcinoma and to study its malignant biological function.

Methods: Western blot, qRT-PCR and immunohistochemistry was used to measure the expression of UBE2Q1 in human lung adenocarcinoma tissues. The association between UBE2Q1 expression and clinic-pathological variables in 99 lung adenocarcinoma samples was analyzed by immunohistochemistry. In vitro experiment, establishing UBE2Q1 knockdown pattern, the markers of apoptosis, cell cycle and epithelial-mesenchymal transition (EMT) were analyzed by Western blot. CCK8, colony formation, Transwell and invasion assay analyzed the effect of UBE2Q1 knockdown on the proliferation, metastasis and invasion of lung cancer cells.

Results: UBE2Q1 was overexpressed in lung adenocarcinoma, and the expression level of UBE2Q1 was related with TNM stage, tumor size, and lymph node metastasis. The high level of UBE2Q1 expression was also associated with poor survival and was an independent risk factor. In vitro, It was also confirmed that steady downregulation of UBE2Q1 could promote apoptosis, induce G2/M cell cycle arrest and regulate EMT. UBE2Q1 silencing dramatically reduce lung tumor cells proliferation, migration and invasion capacities.

Conclusions: UBE2Q1 may serve as a prognostic biomarker and a new therapeutic target of lung adenocarcinoma.

UBE2Q1 作为肺腺癌的新型癌症生物标记物
目的:泛素结合酶(E2s)参与多种肿瘤促进过程。UBE2Q1 是 E2 家族的一员。本研究旨在检测 UBE2Q1 在人肺腺癌中的表达水平,并研究其恶性生物学功能:方法:采用 Western 印迹、qRT-PCR 和免疫组化技术检测 UBE2Q1 在人肺腺癌组织中的表达。免疫组化分析了 99 例肺腺癌样本中 UBE2Q1 表达与临床病理变量之间的关联。在体外实验中,建立 UBE2Q1 基因敲除模式,通过 Western 印迹分析凋亡、细胞周期和上皮-间质转化(EMT)标志物。CCK8、集落形成、Transwell和侵袭实验分析了UBE2Q1敲除对肺癌细胞增殖、转移和侵袭的影响:结果:UBE2Q1在肺腺癌中高表达,其表达水平与TNM分期、肿瘤大小和淋巴结转移有关。UBE2Q1 的高表达水平还与生存率低有关,是一个独立的危险因素。体外实验也证实,稳定下调 UBE2Q1 可促进细胞凋亡、诱导 G2/M 细胞周期停滞和调控 EMT。沉默 UBE2Q1 可显著降低肺肿瘤细胞的增殖、迁移和侵袭能力:结论:UBE2Q1 可作为肺腺癌的预后生物标志物和新的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信