Gene expression levels in cumulus cells are correlated with developmental competence of bovine oocytes

IF 2.4 2区 农林科学 Q3 REPRODUCTIVE BIOLOGY
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Abstract

The generation of mammalian embryos by in vitro culture is hampered by the failure of many of the embryos to develop to the blastocyst stage. This problem occurs even when cumulus–oocyte complexes (COCs) with good morphology are visually selected and used for culture. Because cumulus cells are important for oocyte maturation and subsequent embryo development, here we compared gene expression patterns in cumulus cells of COCs that developed in vitro to the blastocyst stage with those of COCs that failed to develop. Cumulus cells were aspirated from bovine COCs selected for in vitro culture. Oocyte developmental competence was evaluated by screening for cleavage and development to the blastocyst stage. The collected cumulus cells were used to quantify mRNA levels of FSH receptor (FSHR), insulin-like growth factor-1 receptor (IGF-1R), anti-Müllerian hormone (AMH), AMH receptor II (AMHRII), epidermal growth factor receptor (EGFR), estrogen receptor β (ERβ), B cell lymphoma/leukemia-2 associated X (Bax), and cysteine-aspartic acid protease-3 (Caspase-3). We found that the expression levels of FSHR, IGF-1R, AMH, and EGFR were higher in cumulus cells from COCs that developed to blastocysts as compared with those that failed to develop, whereas expression levels of Bax and Caspase-3 were lower in cumulus cells of COCs that matured to the blastocyst stage. Positive correlations were found between FSHR and IGF-1R expression (r = 0.59) and between ERβ and EGFR expression (r = 0.43) in cumulus cells from COCs that developed to the blastocyst stage. Our findings indicate that gene expression levels in cumulus cells are correlated with the developmental competence of bovine oocytes. Measurement of gene expression in cumulus cells therefore offers a non-invasive means of predicting oocyte developmental competence.
积层细胞中的基因表达水平与牛卵母细胞的发育能力相关。
通过体外培养生成哺乳动物胚胎的过程中,许多胚胎无法发育到囊胚阶段。即使目测选择了形态良好的积层-卵母细胞复合体(COC)并用于培养,也会出现这一问题。由于积层细胞对卵母细胞成熟和随后的胚胎发育很重要,我们在此比较了体外发育到囊胚期的 COC 和未能发育的 COC 的积层细胞的基因表达模式。从选作体外培养的牛 COC 中抽取积层细胞。通过筛选卵裂和发育到囊胚期的卵母细胞来评估卵母细胞的发育能力。收集的积层细胞用于量化 FSH 受体 (FSHR)、胰岛素样生长因子-1 受体 (IGF-1R)、抗苗勒氏激素 (AMH)、AMH 受体 II (AMHRII)、表皮生长因子受体 (EGFR)、雌激素受体 β (ERβ)、B 细胞淋巴瘤/白血病-2 相关 X (Bax) 和半胱氨酸-天冬氨酸蛋白酶-3 (Caspase-3) 的 mRNA 水平。我们发现,在发育到囊胚期的 COC 的积层细胞中,FSHR、IGF-1R、AMH 和表皮生长因子受体的表达水平高于发育失败的 COC,而在成熟到囊胚期的 COC 的积层细胞中,Bax 和 Caspase-3 的表达水平较低。在发育到囊胚期的 COC 的积层细胞中,FSHR 和 IGF-1R 的表达呈正相关(r = 0.59),ERβ 和 EGFR 的表达呈正相关(r = 0.43)。我们的研究结果表明,积层细胞中的基因表达水平与牛卵母细胞的发育能力相关。因此,测量积层细胞中的基因表达提供了一种预测卵母细胞发育能力的非侵入性方法。
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来源期刊
Theriogenology
Theriogenology 农林科学-生殖生物学
CiteScore
5.50
自引率
14.30%
发文量
387
审稿时长
72 days
期刊介绍: Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.
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