The supplementation of mannan oligosaccharide in diet promotes the skin wound healing of juvenile turbot, Scophthalmus maximus

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2024-10-09 DOI:10.1016/j.fsi.2024.109953

Jintao Pan , Zhichu Chen , Guijuan Yu , Yaoyao Kong , Qinghui Ai , Kangsen Mai , Yanjiao Zhang

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Abstract

A 30-day feeding trial was conducted to investigate the effects of the supplementation of mannan oligosaccharide (MOS) in the diet on the skin wound healing process of juvenile turbot (Scophthalmus maximus). Two groups of diets were formulated, the control diet (CON) and the control diet supplemented with 0.16 % MOS (MOS), which were fed to the turbot separately. Each group had 3 replicates, with 20 fish per replicate. At the end of the feeding trial, all the fish were weighed and counted. Then four fish per tank were randomly selected for sampling, and the skin of the rest fish was wounded by a biopsy punch. The wounded fish continued to be fed as usual with the same diets respectively, and then sampled again at the 1, 3, and 7 day(s) post wounding (dpw). The results by image analysis showed that the wound closure rate of wounded fish was significantly improved by the supplementation of dietary MOS. As for the results of gene expression, dietary MOS promoted the expression of pro-inflammatory factors (il-1β & tnf-α) and decreased the expression of anti-inflammatory factors (tgf-β1 & il-10). It also enhanced the expression of genes related to re-epithelialization (mmp-9, fgf2, tgf-β1, rock1), as well as new tissue formation and remodeling (fn1, lamb2, col1-α, vegf). Furthermore, dietary MOS promoted re-epithelialization, cell proliferation, collagen deposition, and angiogenesis according to the histomorphological observation. In addition, the supplementation of MOS modified the communities of skin microbiota, decreasing the abundance of Rolstonia, Pseudomonas, and Aeromonas, while increasing the abundance of Pseudoalteromonas luteoviolacea and Shewanella colwellianav. In conclusion, the supplementation of dietary MOS (0.16 %) can promote the re-epithelialization and the recruitment of inflammatory cells, stimulate ECM biosynthesis and angiogenesis, modify the communities of skin microbiota, and ultimately promote the skin wound healing process.

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补充甘露寡糖促进大菱鲆幼鱼皮肤伤口愈合

为了研究日粮中添加甘露寡糖（MOS）对大菱鲆幼鱼皮肤伤口愈合过程的影响，我们进行了为期30天的饲养试验。试验配制了两组日粮，分别为对照日粮（CON）和添加0.16%甘露寡糖的对照日粮（MOS），分别饲喂大菱鲆。每组 3 个重复，每个重复 20 尾鱼。投喂试验结束后，对所有鱼进行称重和计数。然后每缸随机抽取 4 条鱼进行取样，用活检打孔器刺伤其余鱼的皮肤。受伤的鱼继续喂食相同的饲料，然后在受伤后 1 天、3 天和 7 天（dpw）再次取样。图像分析结果表明，添加 MOS 后，受伤鱼的伤口闭合率明显提高。在基因表达方面，膳食 MOS 促进了促炎因子（il-1β 和 tnf-α）的表达，降低了抗炎因子（tgf-β1 和 il-10）的表达。它还提高了与再上皮化（mmp-9、fgf2、tgf-β1、rock1）以及新组织形成和重塑（fn1、lamb2、col1-α、vegf）有关的基因的表达。此外，根据组织形态学观察，膳食 MOS 还能促进再上皮化、细胞增殖、胶原沉积和血管生成。此外，补充 MOS 改变了皮肤微生物群落，降低了罗氏菌、假单胞菌和气单胞菌的丰度，而增加了假交替单胞菌（Pseudoalteromonas luteoviolacea）和雪旺菌（Shewanella colwellianav）的丰度。总之，补充 MOS（0.16%）可以促进皮肤再上皮化和炎症细胞的招募，刺激 ECM 的生物合成和血管生成，改变皮肤微生物群落，最终促进皮肤伤口愈合过程。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.