LncRNA PCBP1-AS1 suppresses cell growth in oral squamous cell carcinoma by targeting miR-34c-5p/ZFP36 axis.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-08 DOI:10.14715/cmb/2024.70.9.14

Orkideh Shafiee Allaf, Wenhao Li, Chongmai Zeng, Peiru Li, Yating Zhang, Yue Xu, Baicheng Bao

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引用次数: 0

Abstract

Oral squamous cell carcinoma (OSCC) is the most frequently diagnosed oral malignancy and poses a great threat to public health. According to bioinformatics analysis, long noncoding RNA PCBP1-AS1 is downregulated in OSCC. In this work, the functions and mechanism of PCBP1-AS1 in OSCC were further investigated. PCBP1-AS1 expression in OSCC cells was measured by quantitative polymerase chain reaction. Cell viability and proliferation were detected using CCK-8 assays and colony-forming assays. TUNEL assays as well as flow cytometry analyses were carried out to detect OSCC cell apoptosis. Binding relationship between PCBP1-AS1 and miR-34c-5p or that between miR-34c-5p and ZFP36 in OSCC cells was identified using RNA immunoprecipitation assays, RNA pulldown assays, and luciferase reporter assays. Experimental results revealed that PCBP1-AS1 was downregulated in OSCC cells. PCBP1-AS1 overexpression hampered cell proliferation and enhanced cell apoptosis in OSCC. PCBP1-AS1 interacted with miR-34c-5p in OSCC and negatively regulated miR-34c-5p. ZFP36 3'untranslated region was targeted by miR-34c-5p. PCBP1-AS1 positively regulated ZFP36 expression. ZFP36 silencing abrogated the suppressive impact of PCBP1-AS1 on OSCC cell growth. In summary, PCBP1-AS1 suppresses cell growth in OSCC by upregulating ZFP36 through interaction with miR-34c-5p.

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LncRNA PCBP1-AS1 通过靶向 miR-34c-5p/ZFP36 轴抑制口腔鳞状细胞癌细胞生长。

口腔鳞状细胞癌（OSCC）是最常见的口腔恶性肿瘤，对公众健康构成巨大威胁。根据生物信息学分析，长非编码 RNA PCBP1-AS1 在 OSCC 中出现下调。本研究进一步探讨了PCBP1-AS1在OSCC中的功能和机制。PCBP1-AS1在OSCC细胞中的表达通过定量聚合酶链反应进行检测。使用 CCK-8 检测法和集落形成检测法检测细胞活力和增殖。TUNEL 试验和流式细胞术分析用于检测 OSCC 细胞凋亡。利用 RNA 免疫沉淀实验、RNA pulldown 实验和荧光素酶报告实验确定了 OSCC 细胞中 PCBP1-AS1 与 miR-34c-5p 之间或 miR-34c-5p 与 ZFP36 之间的结合关系。实验结果显示，PCBP1-AS1 在 OSCC 细胞中被下调。PCBP1-AS1的过表达阻碍了OSCC细胞的增殖，并增强了细胞的凋亡。PCBP1-AS1在OSCC中与miR-34c-5p相互作用，并负向调节miR-34c-5p。ZFP36的3'非翻译区是miR-34c-5p的靶标。PCBP1-AS1正向调节ZFP36的表达。ZFP36 的沉默减弱了 PCBP1-AS1 对 OSCC 细胞生长的抑制作用。综上所述，PCBP1-AS1 通过与 miR-34c-5p 相互作用上调 ZFP36，从而抑制 OSCC 细胞的生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.