Electrochemical/fluorescent dual-mode aptasensor based on 3D porous AuNPs/MXene for detection of ultra-trace mercury (Hg2+)

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2024-10-05 DOI:10.1016/j.bioelechem.2024.108833

Luo Li , Xiaoyi Yan , Yu Liu , Yue Xing , Puyu Zhao , Yunxue Zhu , Ning Liu , Kaiping Sun , Zhiquan Zhang , Shengyong Zhai

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引用次数: 0

Abstract

In this work, the dual-mode aptasensor based on 3D porous AuNPs/MXene using “turn-on” electrochemical method and “turn-off” fluorescent strategy was fabricated. Here, 2D MXene was processed into 3D porous MXene by sacrificial polymethylmethacrylate (PMMA) spherical template. And the meteor hammer-like AuNPs which had good electrochemical properties and quenching effect on fluorescence was synthesized by single electrodeposition. Dual-signal labeled Nile Blue (NB) was in situ grafted to the Hg²⁺ aptamer ends of 3D porous AuNPs/MXene/GCE, and an efficient and sensitive signal interface was constructed to realize the sensitive detection of Hg²⁺. 3D porous AuNPs/MXene had the advantages of large specific surface area, excellent electron transmission performance and signal amplification. The experimental results indicated that this sensor exhibited high sensitivity to Hg²⁺ in both electrochemical and fluorescent sensing, with detection limits of 2.69 fM and 1.60 fM, respectively. Further, the dual-mode aptasensor can ensure the detection accuracy and target quantization. The dual-mode aptasensor has been successfully applied to the ultra-trace detection of Hg²⁺ in actual water samples, which shows the potential of aptamer sensor in detecting heavy metal ions in environmental monitoring.

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基于三维多孔 AuNPs/MXene 的电化学/荧光双模式灵敏传感器，用于检测超痕量汞 (Hg2+)。

本研究采用 "开启 "电化学方法和 "关闭 "荧光策略，制备了基于三维多孔 AuNPs/MXene 的双模式灵敏传感器。在这里，二维 MXene 通过牺牲聚甲基丙烯酸甲酯（PMMA）球形模板加工成三维多孔 MXene。通过单次电沉积合成了具有良好电化学性能和荧光淬灭效果的流星锤状 AuNPs。将双信号标记的尼罗蓝（NB）原位接枝到三维多孔 AuNPs/MXene/GCE 的 Hg2+ 通感末端，构建了高效灵敏的信号接口，实现了对 Hg2+ 的灵敏检测。三维多孔 AuNPs/MXene/GCE 具有比表面积大、电子传输性能好和信号放大等优点。实验结果表明，该传感器在电化学传感和荧光传感中均表现出对 Hg2+ 的高灵敏度，检测限分别为 2.69 fM 和 1.60 fM。此外，该双模式传感器还能确保检测精度和目标量化。该双模适配体传感器已成功应用于实际水样中 Hg2+ 的超痕量检测，显示了适配体传感器在环境监测中检测重金属离子的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.