A WUSCHEL-related homeobox transcription factor, SlWOX4, negatively regulates drought tolerance in tomato.

IF 5.3 2区生物学 Q1 PLANT SCIENCES

Plant Cell Reports Pub Date : 2024-10-06 DOI:10.1007/s00299-024-03333-5

Hui Li, Wanying Ma, Xiao Wang, Hongling Hu, Lina Cao, Hui Ma, Jingwei Lin, Ming Zhong

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引用次数: 0

Abstract

Key message: CRISPR/Cas9-mediated knockout of SlWOX4 gene in tomato enhances tolerance to drought stress. Drought stress is one of the major abiotic factors that seriously affects plant growth and crop yield. WUSCHEL-related homeobox (WOX) transcription factors are involved in plant growth, development and stress response. However, little is known about the role of WOX genes in drought tolerance in tomato. Here, SlWOX4, a member of the WOX family in tomato, was functionally characterized in mediating drought tolerance. SlWOX4 was homologous to Nicotiana tabacum NtWOX4 with a conserved HD domain, and was localized in the nucleus. SlWOX4 was significantly down-regulated by drought and abscisic acid (ABA) treatments. The loss-of-function mutations of SlWOX4 produced using the CRISPR-Cas9 system in tomato improved drought tolerance by reducing water loss rate and enhancing stomatal closure. In addition, the wox4 lines exhibited reduced accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), increased antioxidant enzyme activity, proline contents and ABA contents under drought stress. Moreover, gene editing of SlWOX4 in tomato enhanced drought tolerance by regulating the expression of genes encoding antioxidants and ABA signaling molecules. In summary, SlWOX4 gene might negatively regulate drought stress tolerance in tomato and has great potential as a drought-resistant crop-breeding target genes.

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与 WUSCHEL 相关的同源转录因子 SlWOX4 负向调节番茄的耐旱性。

关键信息：CRISPR/Cas9 介导的番茄 SlWOX4 基因敲除可增强对干旱胁迫的耐受性。干旱胁迫是严重影响植物生长和作物产量的主要非生物因素之一。WUSCHEL 相关同源框（WOX）转录因子参与植物的生长、发育和胁迫响应。然而，人们对 WOX 基因在番茄耐旱性中的作用知之甚少。本文研究了番茄 WOX 家族成员 SlWOX4 在介导耐旱性方面的功能特征。SlWOX4 与烟草 NtWOX4 同源，具有保守的 HD 结构域，定位于细胞核中。SlWOX4在干旱和脱落酸（ABA）处理下明显下调。利用CRISPR-Cas9系统在番茄中产生的SlWOX4功能缺失突变通过降低失水率和提高气孔关闭率来提高耐旱性。此外，在干旱胁迫下，wox4 株系的活性氧（ROS）和丙二醛（MDA）积累减少，抗氧化酶活性、脯氨酸含量和 ABA 含量增加。此外，在番茄中对SlWOX4进行基因编辑可通过调节编码抗氧化剂和ABA信号分子的基因的表达来增强抗旱性。综上所述，SlWOX4基因可能负向调控番茄的干旱胁迫耐受性，有望成为抗旱作物育种的目标基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Cell Reports 生物-植物科学

CiteScore

10.80

自引率

1.60%

发文量

135

审稿时长

3.2 months

期刊介绍： Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.