Vaginal microbiome of women with premature ovarian insufficiency: a descriptive cross-sectional study.

IF 2.9 4区医学 Q1 OBSTETRICS & GYNECOLOGY

Climacteric Pub Date : 2024-10-07 DOI:10.1080/13697137.2024.2393140

Helena Patricia Giraldo, Paulo César Giraldo, Ticiana A Mira, Gabriela Pravatta Rezende, Daniela Angerame Yela, Rose Luce Gomes do Amaral, Cristina Laguna Benetti-Pinto

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Abstract

Objective: This study aimed to describe the vaginal microbiome of women with premature ovarian insufficiency (POI) receiving systemic hormone therapy (HT).

Methods: Forty women with POI receiving systemic HT for at least 6 months, who were sexually active, were included in the descriptive cross-sectional study. Vaginal secretion was collected for DNA extraction followed by pyrosequencing of the 16S rRNA. The samples were pooled into phylogenetic groups (Ravel groups I-V).

Results: Women had mean age of 37.13 (± 7.27) years and POI diagnosis at age 27.90 (± 8.68) years, and a mean HT duration of 8.20 (± 8.73) years. It was observed that 33.4% of the women presented group I flora, with a predominance of Lactobacillus crispatus; 9% group II flora, with a predominance of Lactobacillus gasseri; 33.4% group III flora, with a predominance of Lactobacillus iners; 15.2% group IV flora, with a predominance of anaerobic bacteria; and 9% group V flora, with a predominance of Lactobacillus jensenii.

Conclusion: Women with POI receiving HT presented a vaginal microbiome with a predominance of lactobacilli in the composition of the vaginal flora, specifically L. crispatus and L. iners when evaluated by molecular biology through pyrosequencing of 16S rRNA.

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卵巢早衰妇女的阴道微生物组：一项描述性横断面研究。

研究目的本研究旨在描述接受系统性激素治疗（HT）的卵巢早衰（POI）妇女的阴道微生物组：方法：40 名接受系统性激素治疗至少 6 个月且性生活活跃的卵巢早衰女性被纳入描述性横断面研究。采集阴道分泌物进行 DNA 提取，然后对 16S rRNA 进行热测序。结果显示，妇女的平均年龄为 37.13 岁：妇女的平均年龄为 37.13 (± 7.27)岁，POI 诊断年龄为 27.90 (± 8.68)岁，平均 HT 持续时间为 8.20 (± 8.73)年。观察发现，33.4%的妇女体内存在 I 组菌群，主要是脆片乳杆菌；9%的妇女体内存在 II 组菌群，主要是加塞乳杆菌；33.4%的妇女体内存在 III 组菌群，主要是内斯乳杆菌；15.2%的妇女体内存在 IV 组菌群，主要是厌氧菌；9%的妇女体内存在 V 组菌群，主要是简森乳杆菌：结论：通过 16S rRNA 热测序进行分子生物学评估，患有 POI 并接受 HT 治疗的妇女的阴道微生物群在阴道菌群组成中以乳酸杆菌为主，特别是 L. crispatus 和 L. iners。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Climacteric 医学-妇产科学

CiteScore

1.70

自引率

7.10%

发文量

审稿时长

1 months

期刊介绍： Climacteric is the official journal of the International Menopause Society (IMS). As an international peer-reviewed journal it publishes original research and reviews of all aspects of aging in women. Climacteric was founded by the IMS in 1998 and today has become a leading journal in the publication of peer-reviewed papers on the menopause, climacteric and mid-life health. Topics covered include endocrine changes, symptoms attributed to the menopause and their treatment, hormone replacement and alternative therapies, lifestyles, and the counselling and education of peri- and postmenopausal women. Climacteric, published bimonthly, also features regular invited reviews, editorials and commentaries on recent developments. The editorial review board of Climacteric includes leading scientific and clinical experts in the field of midlife medicine and research and is headed by its Editor-in-Chief, Professor Rod Baber of Australia. He and his team of Associate Editors act independently to set a clear editorial policy, co-ordinate peer review, and ensure a rapid response to submitted papers.