Discovery and characterization of novel lipopeptides produced by Virgibacillus massiliensis with biosurfactant and antimicrobial activities.

IF 2.6 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-04 DOI:10.1007/s13205-024-04100-9

Badiaa Essghaier, Chahnez Naccache, Houda Ben-Miled, Filomena Mottola, Kamel Ben-Mahrez, Maha Mezghani Khemakhem, Lucia Rocco

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引用次数: 0

Abstract

The study aimed to evaluate the biosurfactants (BSs) production by SM-23 strain of Virgibacillus identified by phenotypical and WGS analysis as Virgibacillus massiliensis. We first demonstrated the lipopeptides production by Virgibacillus massiliensis specie and studied their biochemical and molecular analysis as well as their biological potential. The GC-MS analysis indicated that methyl.2-hyroxydodecanoate was the major fatty acid compound with 33.22%. The maximum BSs production was obtained in LB medium supplemented by 1% olive oil (v/v) at 30 °C and 5% NaCl with 1.92 g/l. The obtained results revealed the significant biosurfactants/bioemulsifier potential compared to triton X100 with E24 of 100%, and an emulsification stability SE of 83%. The lipopeptides types were identified by FTIR analysis. A strong antimicrobial action was observed by the produced lipopeptides by the agar diffusion method against E.coli, K. pneumoniae, S. aureus, Fusarium sp, Alternaria sp, and Phytophtora sp. The complete genome sequencing showed genes involved in the synthesis of multiple compounds identified as amphipathic cyclic lipopeptides such as locillomycin/locillomycin B/locillomycin C and bacillibactin. Our results highlighted significant lipopeptides properties displayed by V. massiliensis that can be exploited to develop a novel strategy in the formulation of natural biocidal and fungicidal agents.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04100-9.

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发现 Massiliensis 维吉巴菌产生的具有生物表面活性剂和抗菌活性的新型脂肽并确定其特性。

本研究旨在评估经表型和 WGS 分析鉴定为大肠维吉杆菌（Virgibacillus massiliensis）的 SM-23 株大肠维吉杆菌（Virgibacillus massiliensis）生产生物表面活性剂（BSs）的情况。我们首先证明了大肠维吉杆菌（Virgibacillus massiliensis）能产生脂肽，并研究了其生化和分子分析及其生物潜力。气相色谱-质谱分析表明，2-羟基十二酸甲酯是主要的脂肪酸化合物，占 33.22%。在 30 °C、5%NaCl、添加 1%橄榄油（v/v）的 LB 培养基中，BSs 的产量最大，为 1.92 克/升。研究结果表明，与 E24 为 100%、乳化稳定性 SE 为 83% 的 triton X100 相比，生物表面活性剂/生物乳化剂具有显著的潜力。通过傅立叶变换红外分析确定了脂肽的类型。通过琼脂扩散法，观察到所生产的脂肽对大肠杆菌、肺炎金葡菌、金黄色葡萄球菌、镰刀菌属、交替孢霉属和疫霉属有很强的抗菌作用。全基因组测序显示，有基因参与合成多种被鉴定为两性环脂肽的化合物，如洛钙霉素/洛钙霉素 B/洛钙霉素 C 和巴氏杀菌素。我们的研究结果突显了V. massiliensis所显示的重要脂肽特性，可以利用这些特性开发出一种新型战略，用于配制天然杀菌剂和杀真菌剂：在线版本包含补充材料，可查阅 10.1007/s13205-024-04100-9。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.