Soft surface-enhanced Raman scattering sensing platform based on an oil-in-water emulsion stabilized by silver nanoparticles

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-09-30 DOI:10.1016/j.colsurfb.2024.114278

Hagai Klein , Raz Cohen , Karthik Ananth Mani , Reut Amar Feldbaum , Avital Ben-Haim , Einat Zelinger , Narsingh R. Nirala , Divagar Muthukumar , Abraham J. Domb , Giorgi Shtenberg , Guy Mechrez

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引用次数: 0

Abstract

Pickering emulsions are excellent candidates for developing soft biosensors utilized for detecting native biomolecules such as peptides and proteins through the Surface-Enhanced Raman Spectroscopy (SERS) transduction mechanism. Here, we have developed a SERS sensor based on oil-in-water Pickering emulsions stabilized by Ag nanoparticles (Ag-NPs) with the Raman active molecule (4-Aminothiphenol, 4ATP) adsorbed to their surface. The structural properties and composition of the Pickering emulsion were tuned to meet the demands of the maximal optical response. Our results show that the obtained SERS signals of the main studied Pickering emulsion (water: oil ratio 7:3, 1 wt% Ag-NPs) outperformed colloidal dispersions with the same Ag-NPs concentration by 10-fold at any studied content of 4ATP. The superior optical response of the Pickering emulsion compared to the colloidal dispersion can thus pave the way for the detection of a large variety of analytes at high sensitivity by a soft sensing device. This study innovates by comparing the SERS signals of Raman-active Ag-NPs when they are assembled at the oil/water interface of an emulsion to the case where the NPs are individually dispersed in the medium. The findings shed light on the edit value of utilizing Raman-active Pickering stabilizers for biosensing applications.

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基于银纳米粒子稳定的水包油乳液的软表面增强拉曼散射传感平台。

皮克林乳液是通过表面增强拉曼光谱（SERS）传导机制开发用于检测肽和蛋白质等原生生物分子的软生物传感器的极佳候选材料。在这里，我们开发了一种基于水包油皮克林乳液的 SERS 传感器，该乳液由银纳米粒子（Ag-NPs）稳定，表面吸附有拉曼活性分子（4-氨基硫代苯酚，4ATP）。我们对 Pickering 乳液的结构特性和成分进行了调整，以满足最大光学响应的要求。我们的研究结果表明，主要研究的皮克林乳液（水油比 7:3，1 wt% Ag-NPs）在任何研究的 4ATP 含量下，其获得的 SERS 信号都比具有相同 Ag-NPs 浓度的胶体分散体强 10 倍。因此，与胶体分散体相比，皮克林乳液具有更优越的光学响应，这为利用软传感设备高灵敏度地检测多种分析物铺平了道路。本研究创新性地比较了拉曼活性 Ag-NPs 在乳液的油/水界面组装时与 NPs 单独分散在介质中时的 SERS 信号。研究结果阐明了利用拉曼活性皮克林稳定剂进行生物传感应用的编辑价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.