Improving survival and growth of caprine preantral follicles cultured in medium commonly used for MSC: Role of oxidative stress regulation and epigenetic changes

IF 1.9 2区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Domestic animal endocrinology Pub Date : 2024-09-19 DOI:10.1016/j.domaniend.2024.106890

Ana F.B. Silva , Laritza F. Lima , Anna C.A. Ferreira , Ariclécio C. Oliveira , Napoleão M.A. Neto , Benner G. Alves , Ana P.R. Rodrigues , Eduardo L. Gastal , Vilceu Bordignon , José R. Figueiredo

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Abstract

This study evaluated the efficiency of in vitro culture of preantral follicles (PAF) in a commonly used medium for mesenchymal stem cell (MSC) culture. Parameters assessed included follicle survival, growth, stromal cell density, levels of reduced thiols and reactive oxygen species, epigenetic changes, cell apoptosis, and mRNA abundance. Caprine ovarian tissues were cultured for 1 or 7 days in either PAF or MSC-common media, with uncultured tissues serving as controls. The MSC medium exhibited increased follicular survival and growth and remodeled stromal density potentially through the regulation of oxidative stress and epigenetic changes compared to the PAF medium. In conclusion, our results highlight the importance of the MSC medium in enhancing follicular survival and growth, changing the stromal cell density, as well as in regulating the medium oxidative stress and epigenetic changes during the in vitro culture of caprine PAF.

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提高在间充质干细胞常用培养基中培养的肾上腺前卵泡的存活率和生长率：氧化应激调节和表观遗传变化的作用。

本研究评估了在间充质干细胞（MSC）常用培养基中体外培养前胚泡（PAF）的效率。评估参数包括卵泡存活、生长、基质细胞密度、还原硫醇和活性氧水平、表观遗传学变化、细胞凋亡和mRNA丰度。黄羊卵巢组织在 PAF 或间叶干细胞普通培养基中培养 1 或 7 天，未培养的组织作为对照。与 PAF 培养基相比，间充质干细胞培养基可通过调节氧化应激和表观遗传学变化提高卵泡存活率和生长率，并重塑基质密度。总之，我们的研究结果凸显了间充质干细胞培养基在提高卵泡存活率和生长率、改变基质细胞密度以及在体外培养黄羊PAF过程中调节培养基氧化应激和表观遗传变化方面的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Domestic animal endocrinology 农林科学-奶制品与动物科学

CiteScore

5.50

自引率

4.80%

发文量

审稿时长

31 days

期刊介绍： Domestic Animal Endocrinology publishes scientific papers dealing with the study of the endocrine physiology of domestic animal species. Those manuscripts utilizing other species as models for clinical or production problems associated with domestic animals are also welcome. Topics covered include: Classical and reproductive endocrinology- Clinical and applied endocrinology- Regulation of hormone secretion- Hormone action- Molecular biology- Cytokines- Growth factors