Immunostaining for Mismatch Repair Complex Proteins Impacts on Clinical-Pathological Characteristics and Prognosis of Adenoid Cystic Carcinoma of Salivary Glands

IF 2.7 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine Pub Date : 2024-10-03 DOI:10.1111/jop.13585

Iana Aragão Magalhães, Gabriella Alves Julião Costa, André Alves Crispim, Thinali Sousa Dantas, Fabrício Bitu Sousa, Maria Júlia Barbosa Bezerra, Paulo Goberlânio de Barros Silva

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引用次数: 0

Abstract

Objective

To evaluate the influence of MMR proteins on clinicopathological characteristics and prognosis of salivary gland adenoid cystic carcinoma (ACC).

Method

The solid pattern of ACC showed lower expression for MSH2 (p = 0.039). Significant imbalance in MSH2/MSH6 immunostaining was observed in all histological patterns (p < 0.001), and imbalance in PMS2/MLH1 immunostaining was observed in the cribriform pattern (p = 0.011). The presence of capsule was associated with high expression of MSH6 (p = 0.019), MLH1 (p = 0.045) and PMS2 (p = 0.009). The absence of cribriform pattern (p = 0.002) and capsule pattern (p = 0.025), as well as low expression for MSH6 (p = 0.006) and PMS2 (p = 0.037) were associated with lower overall survival. In multivariate analysis, loss of MSH2 (p = 0.039) and MLH1 (p = 0.017) were significantly associated with worse overall survival.

Results

Twenty-four ACC were clinical-pathologically evaluated and we perform immunohistochemistry for MSH2, MSH6, PMS2 and MLH1. Percentage counting of positive cells was performed in 10 fields of each histological pattern (cribriform, tubular and solid) and the averages of the 30 fields were considered for evaluation with other clinical-pathological variables (Kruskal-Wallis/Dunn, Friedman/Dunn, chi-square, Log-Rank Mantel-Cox tests and Cox regression; SPSS v20.0, p < 0.05).

Discussion

Salivary glands' ACC shows imbalance of the MMR complex and loss of expression of its components is associated with the overall survival of these patients.

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错配修复蛋白免疫染色对唾液腺腺样囊性癌临床病理特征和预后的影响

摘要评估MMR蛋白对唾液腺腺样囊性癌（ACC）临床病理特征和预后的影响：方法：ACC的实体模型显示MSH2表达较低（p = 0.039）。在所有组织学形态中均可观察到 MSH2/MSH6 免疫染色的显著失衡（p 结果：24 例 ACC 经临床-病理诊断为涎腺腺样囊性癌（ACC）：我们对 24 例 ACC 进行了临床病理评估，并对 MSH2、MSH6、PMS2 和 MLH1 进行了免疫组化。我们对每种组织学形态（楔形、管状和实性）的 10 个视野进行了阳性细胞百分比计数，并将 30 个视野的平均值与其他临床病理变量一起进行评估（Kruskal-Wallis/Dunn、Friedman/Dunn、chi-square、Log-Rank Mantel-Cox 检验和 Cox 回归；SPSS v20.0，P 讨论）：唾液腺 ACC 显示 MMR 复合物失衡，其成分表达的丧失与这些患者的总生存率有关。

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来源期刊

Journal of Oral Pathology & Medicine 医学-病理学

CiteScore

5.90

自引率

6.10%

发文量

121

审稿时长

4-8 weeks

期刊介绍： The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.