Acupuncture improves spatial learning and memory impairment caused by herpes simplex virus type-1 in rats through the p38 MAPK/CREB pathway.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-10-03 DOI:10.1186/s12576-024-00941-4

Hongjiao Jin, Rui Huang, Zhu Li, Mi Liu, Ning Zhao, Haiyan Zhang, Yong Lin

{"title":"Acupuncture improves spatial learning and memory impairment caused by herpes simplex virus type-1 in rats through the p38 MAPK/CREB pathway.","authors":"Hongjiao Jin, Rui Huang, Zhu Li, Mi Liu, Ning Zhao, Haiyan Zhang, Yong Lin","doi":"10.1186/s12576-024-00941-4","DOIUrl":null,"url":null,"abstract":"Background: Acupuncture can improve herpes simplex encephalitis (HSE), but the underlying mechanism is not clear. Therefore, we evaluated the cognitive function and apoptosis in hippocampus caused by herpes simplex virus type-1 (HSV-1) in rats after acupuncture and described the molecular mechanism.Methods: Sprague-Dawley rats were induced into HSE models by HSV-1 infection. After 3 days, they received acupuncture at the acupoints of Xuanzhong (GB39), Baihui (GV20), Shenmen (HT7), Shenting (GV24), and Sanyinjiao (SP6), and/or intraperitoneal injection of the p38 MAPK inhibitor SB203580. Morris water maze test was performed on rats. The hippocampus of rats was obtained, and the expression of apoptosis-related genes in the tissues was detected by qRT-PCR. In addition, apoptosis-related proteins and proteins related to the p38 MAPK/CREB pathway in the tissues was detected by western blot.Results: After HSV-1 induction, the rat's escape latency was increased, the time spent on the platform in the target quadrant and the number of platform crossings significantly decreased. In addition, there was an increase in apoptosis in the hippocampus, accompanied by elevated levels of p-p38 and decreased levels of p-CREB. However, these effects could be improved by acupuncture treatment. Interestingly, SB203580 plays a similar role to acupuncture, and acupuncture could further enhance the impacts of SB203580 on cognitive function and apoptosis in hippocampus in HSE rats.Conclusion: Acupuncture improves spatial learning and memory impairment caused by HSV-1 in rats. The functional mechanism of acupuncture may be through the p38 MAPK/CREB pathway.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11448188/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12576-024-00941-4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Acupuncture can improve herpes simplex encephalitis (HSE), but the underlying mechanism is not clear. Therefore, we evaluated the cognitive function and apoptosis in hippocampus caused by herpes simplex virus type-1 (HSV-1) in rats after acupuncture and described the molecular mechanism.

Methods: Sprague-Dawley rats were induced into HSE models by HSV-1 infection. After 3 days, they received acupuncture at the acupoints of Xuanzhong (GB39), Baihui (GV20), Shenmen (HT7), Shenting (GV24), and Sanyinjiao (SP6), and/or intraperitoneal injection of the p38 MAPK inhibitor SB203580. Morris water maze test was performed on rats. The hippocampus of rats was obtained, and the expression of apoptosis-related genes in the tissues was detected by qRT-PCR. In addition, apoptosis-related proteins and proteins related to the p38 MAPK/CREB pathway in the tissues was detected by western blot.

Results: After HSV-1 induction, the rat's escape latency was increased, the time spent on the platform in the target quadrant and the number of platform crossings significantly decreased. In addition, there was an increase in apoptosis in the hippocampus, accompanied by elevated levels of p-p38 and decreased levels of p-CREB. However, these effects could be improved by acupuncture treatment. Interestingly, SB203580 plays a similar role to acupuncture, and acupuncture could further enhance the impacts of SB203580 on cognitive function and apoptosis in hippocampus in HSE rats.

Conclusion: Acupuncture improves spatial learning and memory impairment caused by HSV-1 in rats. The functional mechanism of acupuncture may be through the p38 MAPK/CREB pathway.

查看原文本刊更多论文

针灸可通过 p38 MAPK/CREB 通路改善 1 型单纯疱疹病毒导致的大鼠空间学习和记忆损伤。

背景：针灸可以改善单纯疱疹性脑炎（HSE），但其潜在机制尚不清楚。因此，我们评估了针刺后大鼠的认知功能和由单纯疱疹病毒 1 型（HSV-1）引起的海马凋亡，并描述了其分子机制：方法：用HSV-1感染诱导Sprague-Dawley大鼠建立HSE模型。3天后，针刺玄中（GB39）、百会（GV20）、神门（HT7）、神庭（GV24）和三阴交（SP6）穴位，和/或腹腔注射 p38 MAPK 抑制剂 SB203580。对大鼠进行莫里斯水迷宫试验。取大鼠海马组织，通过 qRT-PCR 检测组织中凋亡相关基因的表达。此外，组织中与凋亡相关的蛋白和与 p38 MAPK/CREB 通路相关的蛋白也通过 western blot 进行了检测：结果：HSV-1诱导后，大鼠的逃逸潜伏期延长，在目标象限平台上停留的时间和穿越平台的次数明显减少。此外，海马凋亡增加，p-p38 水平升高，p-CREB 水平降低。然而，针灸治疗可以改善这些影响。有趣的是，SB203580的作用与针灸相似，针灸可进一步增强SB203580对HSE大鼠认知功能和海马细胞凋亡的影响：结论：针灸可改善 HSV-1 导致的大鼠空间学习和记忆损伤。结论：针灸可改善 HSV-1 导致的大鼠空间学习和记忆损伤，其作用机制可能是通过 p38 MAPK/CREB 通路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464