Automated multimodal imaging of Caenorhabditis elegans behavior in multi-well plates.

IF 3.3 3区生物学 Q2 GENETICS & HEREDITY

Genetics Pub Date : 2024-10-03 DOI:10.1093/genetics/iyae158

Hongfei Ji, Dian Chen, Christopher Fang-Yen

引用次数: 0

Abstract

Assays of behavior in model organisms play an important role in genetic screens, drug testing, and the elucidation of gene-behavior relationships. We have developed an automated, high-throughput imaging and analysis method for assaying behaviors of the nematode C. elegans. We use high-resolution optical imaging to longitudinally record the behaviors of 96 animals at a time in multi-well plates, and computer vision software to quantify the animals' locomotor activity, behavioral states, and egg laying events. To demonstrate the capabilities of our system we used it to examine the role of serotonin in C. elegans behavior. We found that egg-laying events are preceded by a period of reduced locomotion, and that this decline in movement requires serotonin signaling. In addition, we identified novel roles of serotonin receptors SER-1 and SER-7 in regulating the effects of serotonin on egg laying across roaming, dwelling, and quiescent locomotor states. Our system will be useful for performing genetic or chemical screens for modulators of behavior.

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在多孔板中对秀丽隐杆线虫的行为进行自动多模式成像。

模式生物的行为测定在基因筛选、药物测试和阐明基因与行为的关系方面发挥着重要作用。我们开发了一种自动化、高通量的成像和分析方法，用于检测线虫秀丽隐杆线虫的行为。我们使用高分辨率光学成像技术在多孔板中一次纵向记录 96 只动物的行为，并使用计算机视觉软件量化动物的运动活动、行为状态和产卵事件。为了展示我们系统的能力，我们用它来研究血清素在秀丽隐杆线虫行为中的作用。我们发现，在产卵事件之前会有一段运动减少的时期，而这种运动的减少需要血清素信号的传递。此外，我们还发现了血清素受体 SER-1 和 SER-7 在调节血清素对漫游、栖息和静止运动状态下产卵的影响方面的新作用。我们的系统将有助于进行行为调节剂的遗传或化学筛选。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetics GENETICS & HEREDITY-

CiteScore

6.90

自引率

6.10%

发文量

177

审稿时长

1.5 months

期刊介绍： GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.