Diagnostic and evaluative efficiency of ⁶⁸Ga-FAPI-04 in skeletal muscle injury.

IF 3.1 3区医学 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

EJNMMI Research Pub Date : 2024-10-02 DOI:10.1186/s13550-024-01147-w

Yiqun Wang, La Li, Hongde Wang, Jin Cheng, Cancan Du, Luzheng Xu, Yifei Fan, Xiaoqing Hu, Yu Yin, Ruimin Wang, Yingfang Ao

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引用次数: 0

Abstract

Background: Skeletal muscles are vital for daily function, yet assessing their injuries remain challenging. We aimed to elucidate the effectiveness of ⁶⁸Ga-FAPI-04 in evaluating skeletal muscle remodeling.

Results: C2C12 cells were subjected to graded H₂O₂ stimulation in vitro, revealing an initial rise and subsequent decline in fibroblast activation protein (FAP) expression as H₂O₂ concentration increased. In vivo, a murine triceps surae injury model was created using various solutions to simulate normal repair, mild repair failure, and severe repair failure. Assessments were conducted on days 1, 3, 7, and 14 using PET, MRI, and ultrasound. With ⁶⁸Ga-FAPI-04, the normal and mild repair failure groups showed significantly higher SUVmax and T/B ratios on day 1 compared to the severe repair failure group. These values gradually decreased in the normal repair group, becoming negligible after day 7. MRI results for the normal repair group showed low to moderate signal intensity by day 7. A clinical study retrospectively evaluated post-hip arthroplasty patient images at intervals of 1 month, 2-3 months, 5-6 months, and over 7 months. In these patients, ¹⁸F-FDG SUVmax and volume remained relatively stable over time, while ⁶⁸Ga-FAPI-04 SUVmax initially increased, then decreased, with a consistent reduction in volume.

Conclusion: In skeletal muscle injuries, FAP demonstrates a distinctive mechanism of action, and ⁶⁸Ga-FAPI-04, in comparison to other tests, more precisely captures alterations in lesion site uptake intensity and volume.

Trial registration: Trial registration: ChiCTR2000041204. Registered 22 December 2020, https://www.chictr.org.cn/showproj.html?proj=66211.

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68Ga-FAPI-04 在骨骼肌损伤中的诊断和评估效率。

背景：骨骼肌对日常功能至关重要，但评估骨骼肌损伤仍具有挑战性。我们旨在阐明 68Ga-FAPI-04 在评估骨骼肌重塑中的有效性：结果：体外对 C2C12 细胞进行分级 H2O2 刺激，结果显示，随着 H2O2 浓度的增加，成纤维细胞活化蛋白（FAP）的表达开始上升，随后下降。在体内，使用各种溶液模拟正常修复、轻度修复失败和严重修复失败，建立了小鼠肱三头肌损伤模型。在第 1、3、7 和 14 天使用 PET、MRI 和超声波进行评估。在 68Ga-FAPI-04 的作用下，正常修复组和轻度修复失败组在第 1 天的 SUVmax 和 T/B 比值明显高于严重修复失败组。正常修复组的这些值逐渐下降，第 7 天后变得可以忽略不计。正常修复组的核磁共振成像结果在第 7 天时显示出低到中等的信号强度。一项临床研究回顾性地评估了髋关节置换术后患者在 1 个月、2-3 个月、5-6 个月和 7 个月以上的图像。在这些患者中，18F-FDG SUVmax 和体积随着时间的推移保持相对稳定，而 68Ga-FAPI-04 SUVmax 最初上升，然后下降，体积持续减少：结论：在骨骼肌损伤中，FAP显示出独特的作用机制，与其他检测方法相比，68Ga-FAPI-04能更精确地捕捉病变部位摄取强度和体积的变化：试验注册试验注册：ChiCTR2000041204。2020年12月22日注册，https://www.chictr.org.cn/showproj.html?proj=66211。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

EJNMMI Research RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING&nb-

CiteScore

5.90

自引率

3.10%

发文量

审稿时长

13 weeks

期刊介绍： EJNMMI Research publishes new basic, translational and clinical research in the field of nuclear medicine and molecular imaging. Regular features include original research articles, rapid communication of preliminary data on innovative research, interesting case reports, editorials, and letters to the editor. Educational articles on basic sciences, fundamental aspects and controversy related to pre-clinical and clinical research or ethical aspects of research are also welcome. Timely reviews provide updates on current applications, issues in imaging research and translational aspects of nuclear medicine and molecular imaging technologies. The main emphasis is placed on the development of targeted imaging with radiopharmaceuticals within the broader context of molecular probes to enhance understanding and characterisation of the complex biological processes underlying disease and to develop, test and guide new treatment modalities, including radionuclide therapy.