Alleviative effects of green-fabricated zinc oxide nanoparticles on acrylamide-induced oxidative and inflammatory reactions in the rat stomach via modulating gastric neuroactive substances and the MiR-27a-5p/ROS/NF-κB axis

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-09-25 DOI:10.1016/j.tice.2024.102574

Yasmina M. Abd-Elhakim , Amany Abdel-Rahman Mohamed , Tarek Khamis , Mohamed M.M. Metwally , Eman S. El-Shetry , Amirah Albaqami , Wedad Mawkili , Manal E. Alosaimi , Badriyah S. Alotaibi , Naira ElAshmouny , Naief Dahran , Ghadi Alsharif , Mai A. Samak

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引用次数: 0

Abstract

Little is known about the effects of acrylamide (AMD) on the stomach. So, this study evaluated the effect of oral AMD exposure (20 mg/kg b.wt) on oxidative status, apoptotic, and inflammatory reactions in rat’s stomach for 60 days. To explore novel targets of AMD toxicity, a more detailed molecular and immune-expression study was performed. Besides, the possible protective effect of green synthesized zinc oxide nanoparticles (G-ZNP) (10 mg/kg b.wt) was explored. The results revealed that AMD significantly provoked oxidative and lipid peroxidative damage of the stomach in terms of increased ROS and MDA but reduced SOD, CAT, GSH, and GSH/GSSG. Additionally, the stomachs of AMD-exposed rats showed a significant increment of PGE2 but reduced NO. Histopathologically, AMD induced a significant increase in PAS stain and the immunoexpression of iNOS and NF-κB in the glandular stomach. A significant upregulation of CART, VACHT, EGFR, caspase-3, NOS-1, and miR-27a-5p was evident in the stomach of the AMD group. Yet, G-ZNP oral dosing significantly rescued the AMD-induced oxidative damage, apoptotic reaction, inflammatory effect, and altered miR-27a-5p and gene expressions in the stomach. Conclusively, these findings demonstrated the efficacy of G-ZNP in protecting against the harmful impacts of acrylamide on stomach tissues.

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绿色制造的纳米氧化锌颗粒通过调节胃神经活性物质和MiR-27a-5p/ROS/NF-κB轴对丙烯酰胺诱导的大鼠胃氧化和炎症反应具有缓解作用

人们对丙烯酰胺（AMD）对胃的影响知之甚少。因此，本研究评估了口服 AMD（20 毫克/千克体重）60 天对大鼠胃部氧化状态、细胞凋亡和炎症反应的影响。为了探索 AMD 毒性的新靶点，还进行了更详细的分子和免疫表达研究。此外，还探讨了绿色合成氧化锌纳米颗粒（G-ZNP）（10 毫克/千克体重）可能产生的保护作用。结果表明，AMD 会明显引发胃的氧化和脂质过氧化损伤，表现为 ROS 和 MDA 增加，但 SOD、CAT、GSH 和 GSH/GSSG 却减少。此外，AMD 暴露大鼠胃中的 PGE2 显著增加，但 NO 却减少了。从组织病理学角度来看，AMD 会导致腺胃中的 PAS 染色以及 iNOS 和 NF-κB 的免疫表达显著增加。在 AMD 组的胃中，CART、VACHT、表皮生长因子受体、caspase-3、NOS-1 和 miR-27a-5p 明显上调。然而，口服 G-ZNP 能明显缓解 AMD 引起的氧化损伤、细胞凋亡反应、炎症效应以及胃中 miR-27a-5p 和基因表达的改变。这些研究结果最终证明了 G-ZNP 在防止丙烯酰胺对胃组织的有害影响方面的功效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464