The Elimination of Viroids through In Vitro Thermotherapy and a Meristem Tip Culture from a New Limonime Hybrid (Citrus x limon var. limon (L.) Burm. f. x Citrus latifolia var. latifolia).

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioTech Pub Date : 2024-09-23 DOI:10.3390/biotech13030037
Virginia Sarropoulou, Katerina Grigoriadou, Varvara I Maliogka, Chrysoula-Lito Sassalou, Vasileios Ziogas
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Abstract

Viruses and viroids pose a significant challenge in citriculture, and their control is crucial for plant health. This study evaluated the effectiveness of in vitro thermotherapy combined with a meristem tip culture for eliminating citrus exocortis viroid (CEVd) and hop stunt viroid (HSVd) from a new limonime hybrid (Citrus x limon var. limon x Citrus latifolia var. latifolia). The elimination success was confirmed by RT-PCR assays. The in vitro elimination rate for CEVd during the shoot proliferation stage (43%) was higher than for HSVd (21%). Accordingly, in the subsequent rooting stage, the in vitro elimination rate for CEVd (50%) was higher than for HSVd (33%). Successful CEVd and HSVd eradication at a 100% rate was confirmed in the ex vitro acclimatized plants in the greenhouse. The study also established an efficient micropropagation protocol. The optimal treatment for in vitro shoot induction was 0.5-2 mg L-1 benzyladenine (BA) + 0.5 mg L-1 gibberellic acid (GA3) + 0.25 mg L-1 naphthalene acetic acid (NAA), while for shoot elongation, it was 0.5 mg L-1 BA + 0.5 mg L-1 kinetin (KIN) + 0.5 mg L-1 GA3 + 0.25 mg L-1 NAA. Rooting was best promoted by 1 mg L-1 NAA. This study provides valuable insights for the mass production of viroid-free propagation material in this new lemon x lime hybrid, contributing to the conservation of genetic resources in citrus breeding programs through the combined application of in vitro thermotherapy and an in vitro meristem tip culture, a novel and highlighted achievement reported for the first time in this study.

通过体外热疗和新柠檬杂交种(Citrus x limon var.
病毒和病毒病在柑橘栽培中是一项重大挑战,对它们的控制对植物健康至关重要。本研究评估了体外热疗法与分生组织尖端培养相结合对消除新柠檬杂交种(Citrus x limon var.通过 RT-PCR 检测确认了消除的成功率。在芽增殖阶段,CEVd 的体外消除率(43%)高于 HSVd(21%)。相应地,在随后的生根阶段,CEVd 的体外消除率(50%)也高于 HSVd(33%)。在温室中的离体适应植物中,CEVd 和 HSVd 的成功根除率达到了 100%。这项研究还确立了一种高效的微繁殖方案。体外芽诱导的最佳处理为 0.5-2 mg L-1 苄基腺嘌呤(BA)+ 0.5 mg L-1 赤霉素(GA3)+ 0.25 mg L-1 萘乙酸(NAA),而芽伸长的最佳处理为 0.5 mg L-1 BA + 0.5 mg L-1 激肽(KIN)+ 0.5 mg L-1 GA3 + 0.25 mg L-1 NAA。1 mg L-1 NAA最能促进生根。这项研究为大规模生产柠檬×青柠杂交种的无病毒繁殖材料提供了有价值的见解,通过体外热疗和体外分生组织尖端培养的联合应用,为柑橘育种项目中遗传资源的保护做出了贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BioTech
BioTech Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
3.70
自引率
0.00%
发文量
51
审稿时长
11 weeks
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