Communication between cancer cell subtypes by exosomes contributes to nasopharyngeal carcinoma metastasis and poor prognosis.

IF 5.1 4区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Precision Clinical Medicine Pub Date : 2024-09-23 eCollection Date: 2024-09-01 DOI:10.1093/pcmedi/pbae018

Hao-Jun Xie, Ming-Jie Jiang, Ke Jiang, Lin-Quan Tang, Qiu-Yan Chen, An-Kui Yang, Hai-Qiang Mai

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引用次数: 0

Abstract

Background: Intratumor heterogeneity is common in cancers, with different cell subtypes supporting each other to become more malignant. Nasopharyngeal carcinoma (NPC), a highly metastatic cancer, shows significant heterogeneity among its cells. This study investigates how NPC cell subtypes with varying metastatic potentials influence each other through exosome-transmitted molecules.

Methods: Exosomes were purified and characterized. MicroRNA expression was analyzed via sequencing and qRT-PCR. The effects of miR-30a-5p on migration, invasion, and metastasis were evaluated in vitro and in vivo. Its impact on desmoglein glycoprotein (DSG2) was assessed using dual-luciferase assays and Western blotting. Immunohistochemistry (IHC) and statistical models linked miR-30a-5p/DSG2 levels to patient prognosis.

Results: Different NPC cell subtypes transmit metastatic potential via exosomes. High-metastatic cells enhance the migration, invasion, and metastasis of low-metastatic cells through exosome-transmitted miR-30a-5p. Plasma levels of exosomal miR-30a-5p are reliable indicators of NPC prognosis. miR-30a-5p may promote metastasis by targeting DSG2 and modulating Wnt signaling. Plasma exosomal miR-30a-5p inversely correlates with DSG2 levels, predicting patient outcomes.

Conclusion: High-metastatic NPC cells can increase the metastatic potential of low-metastatic cells through exosome-transmitted miR-30a-5p, which is a valuable prognostic marker assessable via liquid biopsy.

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癌细胞亚型之间通过外泌体进行交流，导致鼻咽癌转移和预后不良。

背景：肿瘤内异质性在癌症中很常见，不同的细胞亚型相互支持，恶性程度更高。鼻咽癌（NPC）是一种高度转移性癌症，其细胞间存在显著的异质性。本研究探讨了具有不同转移潜能的鼻咽癌细胞亚型如何通过外泌体传递的分子相互影响：方法：纯化并鉴定外泌体。通过测序和 qRT-PCR 分析了微 RNA 的表达。在体外和体内评估了 miR-30a-5p 对迁移、侵袭和转移的影响。使用双荧光素酶测定法和 Western 印迹法评估了 miR-30a-5p 对去甲斑鸠蛋白糖蛋白（DSG2）的影响。免疫组织化学（IHC）和统计模型将 miR-30a-5p/DSG2 水平与患者预后联系起来：结果：不同的鼻咽癌细胞亚型通过外泌体传递转移潜能。结果：不同的鼻咽癌细胞亚型通过外泌体传递转移潜能。高转移细胞通过外泌体传递的 miR-30a-5p 增强低转移细胞的迁移、侵袭和转移。miR-30a-5p可能通过靶向DSG2和调节Wnt信号来促进转移。血浆外泌体miR-30a-5p与DSG2水平成反比，可预测患者的预后：结论：高转移性鼻咽癌细胞可通过外泌体传递的miR-30a-5p增加低转移性细胞的转移潜能，而miR-30a-5p是一种有价值的预后标志物，可通过液体活检进行评估。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Precision Clinical Medicine MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

10.80

自引率

0.00%

发文量

审稿时长

5 weeks

期刊介绍： Precision Clinical Medicine (PCM) is an international, peer-reviewed, open access journal that provides timely publication of original research articles, case reports, reviews, editorials, and perspectives across the spectrum of precision medicine. The journal's mission is to deliver new theories, methods, and evidence that enhance disease diagnosis, treatment, prevention, and prognosis, thereby establishing a vital communication platform for clinicians and researchers that has the potential to transform medical practice. PCM encompasses all facets of precision medicine, which involves personalized approaches to diagnosis, treatment, and prevention, tailored to individual patients or patient subgroups based on their unique genetic, phenotypic, or psychosocial profiles. The clinical conditions addressed by the journal include a wide range of areas such as cancer, infectious diseases, inherited diseases, complex diseases, and rare diseases.