Application of loop-mediated isothermal amplification (LAMP) in plant pathogen detection.

IF 2.6 2区 农林科学 Q2 PLANT SCIENCES
Liuliu Yang, Yu Sun, Lin Sun, Zehao Wang, Jie Feng, Yue Liang
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引用次数: 0

Abstract

Plant diseases impact the production of all kinds of crops, resulting in significant economic losses worldwide. Timely and accurate detection of plant pathogens is crucial for surveillance and management of plant diseases. In recent years, loop-mediated isothermal amplification (LAMP) has become a popular method for pathogen detection and disease diagnosis due to the advantages of its simple instrument requirement and constant reaction temperature. In this review, we provide an overview of current research on LAMP, including the reaction system, design of primers, selection of target regions, visualization of amplicons, and application of LAMP on the detection of all major groups of plant pathogens. We also discuss plant pathogens for which LAMP is yet to be developed, potential improvements of plant disease diagnosis, and disadvantages that need to be considered.

环介导等温扩增(LAMP)在植物病原体检测中的应用。
植物病害影响着各种农作物的生产,给全世界造成了巨大的经济损失。及时准确地检测植物病原体对于植物病害的监控和管理至关重要。近年来,环介导等温扩增法(LAMP)因其仪器要求简单、反应温度恒定等优点,已成为病原体检测和病害诊断的常用方法。在这篇综述中,我们概述了目前有关 LAMP 的研究,包括反应系统、引物设计、靶区选择、扩增子可视化以及 LAMP 在检测所有主要植物病原体类群中的应用。我们还讨论了 LAMP 尚待开发的植物病原体、植物病害诊断的潜在改进以及需要考虑的缺点。
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来源期刊
Phytopathology
Phytopathology 生物-植物科学
CiteScore
5.90
自引率
9.40%
发文量
505
审稿时长
4-8 weeks
期刊介绍: Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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