The PATROL1 function in roots contributes to the increase in shoot biomass.

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-09-26 DOI:10.1007/s00425-024-04526-8
Michitaka Notaguchi, Manami Ichita, Takaya Kawasoe, Keina Monda, Ken-Ichi Kurotani, Takumi Higaki, Koh Iba, Mimi Hashimoto-Sugimoto
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引用次数: 0

Abstract

Main conclusion: PATOL1 contributes to increasing biomass not only by effective stomatal movement but also by root meristematic activity. PATROL1 (PROTON ATPase TRANSLOCATION CONTROL 1), a protein with a MUN domain, is involved in the intercellular trafficking of AHA1 H+-ATPase to the plasma membrane in guard cells. This allows for larger stomatal opening and more efficient photosynthesis, leading to increased biomass. Although PATROL1 is expressed not only in stomata but also in other tissues of the shoot and root, the role in other tissues than stomata has not been determined yet. Here, we investigated PATROL1 functions in roots using a loss-of-function mutant and an overexpressor. Cytological observations revealed that root meristematic size was significantly smaller in the mutant resulting in the short primary root. Grafting experiments showed that the shoot biomass of the mutant scion was increased when it grafted onto wild-type or overexpressor rootstocks. Conversely, grafting of the overexpressor scion shoot enhanced the growth of the mutant rootstock. The leaf temperatures of the grafted plants were consistent with those of their respective genotypes, indicating cell-autonomous behavior of stomatal movement and independent roles of PATROL1 in plant growth. Moreover, plasma membrane localization of AHA1 was not altered in root epidermal cells in the patrol1 mutant implying existence of a different mode of PATROL1 action in roots. Thus PATROL1 plays a role in root meristem and contributes to increase shoot biomass.

根部的 PATROL1 功能有助于增加芽的生物量。
主要结论PATOL1 不仅能通过有效的气孔运动,还能通过根分生组织活动促进生物量的增加。PATROL1(PROTON ATPase TRANSLOCATION CONTROL 1)是一种具有 MUN 结构域的蛋白质,它参与了 AHA1 H+-ATPase 向保卫细胞质膜的胞间转移。这使得气孔开度更大,光合作用效率更高,从而增加了生物量。虽然 PATROL1 不仅在气孔中表达,在芽和根的其他组织中也有表达,但其在气孔以外的其他组织中的作用尚未确定。在这里,我们利用功能缺失突变体和过表达器研究了 PATROL1 在根中的功能。细胞学观察发现,突变体的根分生组织尺寸明显变小,导致主根变短。嫁接实验表明,当突变体接穗嫁接到野生型或过表达型砧木上时,其芽的生物量会增加。相反,过表达接穗芽的嫁接会促进突变体砧木的生长。嫁接植株的叶片温度与各自基因型的叶片温度一致,表明气孔运动是细胞自主行为,PATROL1在植物生长中发挥独立作用。此外,patrol1 突变体根表皮细胞中 AHA1 的质膜定位并没有改变,这意味着 PATROL1 在根中存在不同的作用模式。因此,PATROL1 在根分生组织中发挥作用,有助于增加芽的生物量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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