Targeted isolation of lignans from the roots of Anthriscus sylvestris (L.) Hoffm. by small molecule accurate recognition technology

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Phytochemistry Pub Date : 2024-09-28 DOI:10.1016/j.phytochem.2024.114293

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Abstract

Five undescribed lignans (1–5), along with sixteen known lignans (6–21), were isolated from the roots of Anthriscus sylvestris using small molecule accurate recognition technology (SMART). The structures of the isolated compounds were determined by comprehensive spectroscopic analyses, and the absolute configurations of compounds 3–5 were elucidated by comparison of their calculated and experimental ECD spectra. Compounds 5, 14−15, 19, and 21 exhibited significantly inhibitory effects against hypoxia-stimulated abnormal proliferation of pulmonary arterial smooth muscle cells (PASMCs). Moreover, compounds 5, 14−15, 19, and 21 can significantly restore expression of expression of PASMCs proliferation-related protein, including α-SMA, PCNA, P27, and CyclinD3, which are closely related to cell proliferation.

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利用小分子精确识别技术，有针对性地从西洋蒽（L. Hoffm.）根中分离木质素。

利用小分子精确识别技术（SMART）从芒柄蜡草（Anthriscus sylvestris）根部分离出了五种未被描述的木质素（1-5）和十六种已知的木质素（6-21）。通过全面的光谱分析确定了分离化合物的结构，并通过比较计算和实验 ECD 光谱阐明了化合物 3-5 的绝对构型。化合物 5、14-15、19 和 21 对缺氧刺激的肺动脉平滑肌细胞（PASMCs）的异常增殖有明显的抑制作用。此外，化合物 5、14-15、19 和 21 还能明显恢复 PASMCs 增殖相关蛋白的表达，包括与细胞增殖密切相关的 α-SMA、PCNA、P27 和 CyclinD3。

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来源期刊

Phytochemistry 生物-植物科学

CiteScore

6.40

自引率

7.90%

发文量

443

审稿时长

39 days

期刊介绍： Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.