Evaluation of the cytotoxic and antioxidant activities Buxus wallichiana Baill leaf extract against U 87 glioblastoma.

Abstract

The scientific community has been drawn towards natural plant resources due to the rising success rates. This work aimed to explore the phytochemical, antioxidant and anti-cancer properties of an ethanolic extract derived from the leaves of Buxus wallichiana Baill. which is utilized in traditional medicine in the treatment of various disease such as respiratory disorders, GI disorders and inflammation. Chemical composition of the ethanolic extract of BW leaves was evaluated by using Gas chromatography-mass spectrometry and phytochemical analysis. Moreover, anti-oxidant and anticancer activity was carried out through 2,2-diphenyl-1-picryl hydrazyl scavenging activity method and 3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay against human glioblastoma cell line. ANOVA test was used to analyze the results followed by Bonferoni's post hoc. Results were significant at P <0.05 and demonstrated that, blocking the production of free radicals also caused tumor cell proliferation on the U 87 MG. Thus, the results show the same pattern of toxicity as indicated by the American National Cancer Institute. The minimum dose for cytotoxic activity of the crude extract was less than 30μg/mL. BW leave extract is considered to have promising anticancer potential as well as antioxidant due to the presence of potent compounds and could become a source of treatment with minimum side effects.