Microevolution of Bartonella grahamii driven by geographic and host factors.

IF 5 2区生物学 Q1 MICROBIOLOGY

mSystems Pub Date : 2024-10-22 Epub Date: 2024-09-30 DOI:10.1128/msystems.01089-24

Ailing Xu, Liang Lu, Wen Zhang, Xiuping Song, Guichang Li, Yu Miao, Ruixiao Li, Min Chen, Qiyong Liu, Dongmei Li

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引用次数: 0

Abstract

Bartonella grahamii is one of the most prevalent Bartonella species in wild rodents and has been associated with human cases of neuroretinitis. The structure and distribution of genomic diversity in natural B. grahamii is largely unexplored. Here, we have applied a comprehensive population genomic and phylogenomic analysis to 172 strains of B. grahamii to unravel the genetic differences and influencing factors that shape its populations. The findings reveal a remarkable genomic diversity within the species, primarily in the form of single- nucleotide polymorphisms. The open pangenome of B. grahamii indicates a dynamic genomic evolution influenced by its ecological niche. Whole-genome data allowed us to decompose B. grahamii diversity into six phylogroups, each characterized by a unique "mosaic pattern" of hosts and biogeographic regions. This suggests a complex interplay between host specificity and biogeography. In addition, our study suggests a possible origin of European strains from Asian lineages, and host factors have a more significant impact on the genetic differentiation of B. grahamii than geographical factors. These insights contribute to understanding the evolutionary history of this pathogen and provide a foundation for future epidemiological research and public health strategies.

Importance: Bartonella grahamii has been reported worldwide and shown to infect humans. Up to now, an effective transmission route of B. grahamii to humans has not been confirmed. The genetic evolution of B. grahamii and the relationship between B. grahamii and its host need to be further studied. The factors driving the genetic diversity of B. grahamii are still controversial. The results showed that the European isolates shared a common ancestor with the Chinese isolates. Host factors were shown to play an important role in driving the genetic diversity of B. grahamii. When host factors were fixed, geographic barriers drove B. grahamii microevolution. Our study emphasizes the importance of characterizing isolate genomes derived from hosts and geographical locations and provides a new reference for the origin of B. grahamii.

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由地理和宿主因素驱动的格雷厄姆巴顿氏菌的微进化。

格雷厄姆巴顿氏菌是野生啮齿类动物中最常见的巴顿氏菌之一，与人类神经视网膜炎病例有关。天然格雷厄姆巴氏杆菌基因组多样性的结构和分布在很大程度上尚未被探索。在这里，我们对 172 株格雷厄姆巴氏杆菌进行了全面的群体基因组和系统发生组分析，以揭示形成其群体的遗传差异和影响因素。研究结果表明，该物种的基因组具有显著的多样性，主要表现为单核苷酸多态性。B. grahamii开放的泛基因组表明其基因组受生态位的影响发生了动态进化。全基因组数据使我们能够将 B. grahamii 的多样性分解为六个系统群，每个系统群都以宿主和生物地理区域的独特 "马赛克模式 "为特征。这表明宿主特异性和生物地理学之间存在复杂的相互作用。此外，我们的研究还表明，欧洲菌株可能起源于亚洲菌系，寄主因素对 B. grahamii 遗传分化的影响比地理因素更大。这些见解有助于了解这种病原体的进化史，并为未来的流行病学研究和公共卫生策略奠定了基础：重要意义：格拉汉姆巴氏杆菌已在全球范围内被报道，并被证明可感染人类。迄今为止，格雷厄姆巴氏杆菌对人类的有效传播途径尚未得到证实。格拉汉姆巴氏杆菌的遗传进化以及格拉汉姆巴氏杆菌与其宿主之间的关系需要进一步研究。目前对导致 B. grahamii 遗传多样性的因素仍存在争议。结果表明，欧洲分离株与中国分离株有共同的祖先。结果表明，宿主因素在驱动 B. grahamii 遗传多样性方面发挥了重要作用。当宿主因素固定时，地理障碍推动了 B. grahamii 的微进化。我们的研究强调了鉴定来自宿主和地理位置的分离株基因组特征的重要性，并为 B. grahamii 的起源提供了新的参考。

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来源期刊

mSystems Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

10.50

自引率

3.10%

发文量

308

审稿时长

13 weeks

期刊介绍： mSystems™ will publish preeminent work that stems from applying technologies for high-throughput analyses to achieve insights into the metabolic and regulatory systems at the scale of both the single cell and microbial communities. The scope of mSystems™ encompasses all important biological and biochemical findings drawn from analyses of large data sets, as well as new computational approaches for deriving these insights. mSystems™ will welcome submissions from researchers who focus on the microbiome, genomics, metagenomics, transcriptomics, metabolomics, proteomics, glycomics, bioinformatics, and computational microbiology. mSystems™ will provide streamlined decisions, while carrying on ASM''s tradition of rigorous peer review.