The Discovery of Membrane Vesicle Biogenesis in the Polyhydroxybutyrate-non-producing Mutant Strain of Cupriavidus necator H16.

IF 2.1 4区环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbes and Environments Pub Date : 2024-01-01 DOI:10.1264/jsme2.ME24007

Sangho Koh, Michio Sato, Hiromi Matsusaki, Seiichi Taguchi

引用次数: 0

Abstract

Extracellular membrane vesicles (MVs) caused by the artificial production of polyhydroxybutyrate (PHB) were previously detected in Escherichia coli. We herein observed MV biogenesis in the mutant strain (-PHB) of the natural PHB producer, Cupriavidus necator H16. This inverse relationship was revealed through comparative electron microscopic ana-lyses of wild-type and mutant strains. Based on these results, we speculate that a physiological relationship exists between MV biogenesis and PHB biosynthesis. Therefore, we propose the potential of MV biogenesis as a fermentative "stress marker" for monitoring the performance of target polymer-producing microbial platforms.

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在不产生多羟基丁酸的突变株中发现膜囊泡的生物生成过程。

以前曾在大肠杆菌中检测到人工生产聚羟基丁酸（PHB）引起的胞外膜囊泡（MV）。我们在此观察了天然 PHB 生产者--坏死葡萄球菌 H16 的突变菌株（-PHB）的 MV 生物生成情况。通过对野生型菌株和突变型菌株进行比较电子显微镜分析，我们发现了这种反向关系。基于这些结果，我们推测 MV 生物生成与 PHB 生物合成之间存在生理关系。因此，我们认为 MV 的生物发生有可能成为一种发酵 "压力标记"，用于监控目标聚合物生产微生物平台的性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbes and Environments 生物-生物工程与应用微生物

CiteScore

4.10

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.