Purification, Source Exploration, and Bioactivity Evaluation of Oligosaccharides in Sijunzi Decoction

IF 2.8 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL
Xiaonan Chen, Zongjin Pu, Yixin Liu, Bangjian Dong, Xiaobo Li
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引用次数: 0

Abstract

Sijunzi decoction (SJZD) has been widely used to treat splenic deficiency syndrome. Previous studies confirmed that polysaccharides and non-polysaccharides (NPS) are the main active components of SJZD. This study aimed to investigate the composition and activity of oligosaccharides in NPS. In this study, the oligosaccharide component (named SJZD-OGS), consisting of several different oligosaccharides, was separated and purified from non-polysaccharides of SJZD (SJZD_NPS). Ultra-high-performance liquid chromatography–ion mobility spectrometry–quadrupole time-of-flight mass spectrometry (UHPLC–IMS–QTOF/MS), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) were used to analyze SJZD-OGS. SJZD-OGS, with a molecular weight of 2178 Da, was composed of glucose and fructose, and its chemical composition mainly included fructo-oligosaccharides (FOS) with a degree of polymerization of 3–14 (DP 3–14). The results of oligosaccharide source exploration demonstrated that the chemical composition of SJZD-OGS was different from that of the oligosaccharides of each herb in SJZD. In addition, SJZD-OGS showed good anti-inflammatory activity on macrophage cells and intestinal epithelial cell protective activity. The present work provides experimental evidence for elucidating the pharmaceutical components of SJZD and presents an effective strategy for the research of oligosaccharides in traditional Chinese medicine (TCM).

四君子汤中低聚糖的纯化、探源及生物活性评价
四君子汤(SJZD)被广泛用于治疗脾虚综合征。以往的研究证实,多糖和非多糖(NPS)是四君子汤的主要活性成分。本研究旨在探讨 NPS 中寡糖的组成和活性。本研究从 SJZD 的非多糖(SJZD_NPS)中分离纯化了由几种不同寡糖组成的寡糖成分(命名为 SJZD-OGS)。采用超高效液相色谱-离子迁移谱-四极杆飞行时间质谱法(UHPLC-IMS-QTOF/MS)、基质辅助激光解吸/电离飞行时间质谱法(MALDI-TOF-MS)和高性能阴离子交换色谱-脉冲安培检测法(HPAEC-PAD)分析 SJZD-OGS。SJZD-OGS的分子量为2178 Da,由葡萄糖和果糖组成,其化学成分主要包括聚合度为3-14(DP 3-14)的果寡糖(FOS)。低聚糖的探源结果表明,SJZD-OGS 的化学成分与 SJZD 中各药材的低聚糖不同。此外,SJZD-OGS 对巨噬细胞具有良好的抗炎活性和肠上皮细胞保护活性。本研究为阐明 SJZD 的药物成分提供了实验证据,并为低聚糖在中药中的研究提供了有效策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of separation science
Journal of separation science 化学-分析化学
CiteScore
6.30
自引率
16.10%
发文量
408
审稿时长
1.8 months
期刊介绍: The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.
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