Potential and Metabolic Impacts of Double Enrichments of Docosahexaenoic Acid and 25-Hydroxy Vitamin D3 in Tissues of Broiler Chickens

IF 3.7 3区医学 Q2 NUTRITION & DIETETICS

Journal of Nutrition Pub Date : 2024-11-01 DOI:10.1016/j.tjnut.2024.09.022

Sahil Kalia , Andrew D Magnuson , Tao Sun, Ziqiao Sun, Xin Gen Lei

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引用次数: 0

Abstract

Background

Chicken may be enriched with 25-hydroxy D₃ [25(OH)D₃] and docosahexaenoic acid (DHA) to enhance the dietary intake of the public.

Objectives

Two experiments (Expt.) were conducted to determine the potential and metabolic impacts of enriching both DHA and 25(OH)D₃ in the tissues of broiler chickens.

Methods

In Expt. 1, 144 chicks (6 cages/treatment and 6 birds/cage) were fed a corn–soybean meal basal diet (BD), BD + 10,000 IU 25(OH)D₃/kg [BD + 25(OH)D₃], BD + 1% DHA-rich Aurantiochytrium (1.2 g DHA/kg; BD + DHA), or BD + 25(OH)D₃+DHA for 6 wk. In Expt. 2, 180 chicks were fed the BD, BD + DHA-rich microalgal oil (1.5–3.0 g DHA/kg, BD + DHA), BD + DHA + eicosapentaenoic acid (EPA)-rich microalgae (0.3–0.6 g EPA/kg, BD + DHA + EPA), BD + DHA + 25(OH)D₃ [6000 to 12,000 IU/kg diet; BD + DHA + 25(OH)D₃], and BD + DHA + EPA + 25(OH)D₃ for 6 wk.

Results

Supranutrition of these 2 nutrients resulted in 57–62 mg DHA and 1.9–3.3 μg of 25(OH)D₃/100 g of breast or thigh muscles. The DHA enrichment was independent of dietary EPA or 25(OH)D₃, but that of 25(OH)D₃ in the liver was decreased (68%, P < 0.05) by dietary DHA in Expt. 1. Compared with BD, BD + 25(OH)D₃ enhanced (P < 0.05) gene expression related to D₃ absorption (scavenger receptor class B type 1 and Niemann-pick c1 like 1) in the liver and D₃ degradation (cytochrome P450 24A1) in the breast, and decreased mRNA or protein concentrations of vitamin D binding protein in the adipose tissue or thigh muscle. Supranutrition of DHA decreased mRNA concentrations of lipid metabolism-related genes (fatty acid desaturase 1,2, ELOVL fatty acid elongase 5, fatty acid desaturase 2, fatty acid synthase, and sterol regulatory element-binding protein 1).

Conclusions

Both DHA and 25(OH)D₃ were enriched in the muscles up to meeting 50%–100% of the suggested intakes of these nutrients by consuming 2 servings of 100 g of fortified chicken. The enrichments altered gene expression related to lipid biosynthesis and vitamin D transport or storage.

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肉鸡组织中双重富集二十二碳六烯酸和 25- 羟基维生素 D3 的潜力和代谢影响。

背景：鸡肉可富含 25- 羟基 D3 [25(OH)D3] 和二十二碳六烯酸 (DHA)，以提高公众的膳食摄入量：进行了两项实验，以确定在肉鸡组织中富含 DHA 和 25(OH)D3 的潜在影响和代谢影响：在试验 1 中，144 只雏鸡（6 个笼子/处理，6 只/笼子）连续 6 周饲喂玉米-豆粕基础日粮（BD）、BD+10,000 IU 25(OH)D3/kg [BD+25(OH)D3]、BD+1% 富含 DHA 的金黄色葡萄球菌（1.2 g DHA/kg；BD+DHA）或 BD+25(OH)D3+DHA。在试验 2 中，给 180 只小鸡喂食 BD、BD+富含 DHA 的微藻油（1.5 至 3.0 克 DHA/千克，BD+DHA）、BD+DHA + 富含二十碳五烯酸（EPA）的微藻（0.3至0.6克EPA/千克，BD+DHA+EPA）、BD+DHA+25(OH)D3[6,000至12,000 IU/千克膳食；BD+DHA+25(OH)D3]和BD+DHA+EPA+25(OH)D3，持续6周：每 100 克胸肌或大腿肌肉中含有 57 至 62 毫克 DHA 和 1.9 至 3.3 微克 25(OH)D3。DHA 的富集与膳食 EPA 或 25(OH)D3 无关，但在试验 1 中，膳食 DHA 会降低肝脏中 25(OH)D3 的含量（68%，P < 0.05）。与BD相比，BD+25(OH)D3会增强（P＜0.05）肝脏中与D3吸收相关的基因表达（SRB1、NPC1L1）和乳腺中与D3降解相关的基因表达（CYP24A1），并降低脂肪组织或大腿肌肉中维生素D结合蛋白的mRNA或蛋白水平。过量摄入 DHA 会降低脂质代谢相关基因（FADS1、2、ELOVL5、FADS2、FASN 和 SREBP1）的 mRNA 水平：通过食用两份 100 克强化鸡肉，肌肉中的 DHA 和 25(OH)D3 均可达到建议摄入量的 50%至 100%。富集改变了与脂质生物合成和维生素 D 运输或储存有关的基因表达。

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来源期刊

Journal of Nutrition 医学-营养学

CiteScore

7.60

自引率

4.80%

发文量

260

审稿时长

39 days

期刊介绍： The Journal of Nutrition (JN/J Nutr) publishes peer-reviewed original research papers covering all aspects of experimental nutrition in humans and other animal species; special articles such as reviews and biographies of prominent nutrition scientists; and issues, opinions, and commentaries on controversial issues in nutrition. Supplements are frequently published to provide extended discussion of topics of special interest.