Sex differences in quantitative ultrasonographic measurements of the rectus femoris in children.

Abstract

The distribution and amount of intramuscular fat and fibrous tissue can be influenced by biological sex and impact muscle quality in both the functional (force-generating capacity) and morphological (muscle composition) domains. While ultrasonography (US) has proven effective in assessing age- or sex-related differences in muscle quality, limited information is available on sex differences in children. Quantitative ultrasonographic measurements, such as echo intensity (EI), EI bands (number of pixels across 50-unit intervals) and texture, may offer a comprehensive framework for identifying sex differences in muscle composition. The aim of our study was to examine the effect of sex on the rectus femoris (RF) muscle quality in children. We used EI (mean and bands) and texture as muscle quality estimates derived from B-mode US. We hypothesised that RF muscle quality differs significantly between girls and boys. Additionally, we also hypothesised that there is a significant correlation between EI bands and texture. Forty-four non-active healthy children were recruited (n = 22 girls, 12.8 ± 1.5 years; and n = 22 boys, 13.5 ± 1.2 years). RF was assessed using EI mean, EI bands, and texture analysis (homogeneity and correlation) using the Gray-Level Co-Occurrence Matrix. The results revealed significant (p < 0.05) sex differences in RF EI bands and texture. Boys displayed higher values in the 0-50 EI band and had more homogeneous muscle texture than girls. Conversely, girls displayed greater values in the 51-100 EI band and had less homogenous texture compared to boys (p < 0.05). A positive correlation was observed between the 0-50 EI band and muscle homogeneity. However, the 51-100 EI band correlated negatively with homogeneity (p < 0.05), particularly for girls. In conclusion, our study revealed sex-specific differences in mean EI, EI bands, and texture of the RF muscle in children. The variations in the correlations between the first and second EI bands and texture reveal different levels of homogeneity in each band. This indicates that distinct muscle tissue constituents, such as intramuscular fat and/or connective tissue, may be reflected in EI bands. Overall, the methods used in this study may be useful for examining muscle quality in healthy children and those with medical conditions.