Microbulbifer spongiae sp. nov., isolated from marine sponge Diacarnus erythraeanus.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Nabila Ishaq, Mimi Zhang, Luyao Gao, Micha Ilan, Zhiyong Li
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Abstract

A novel bacterium, designated as MI-GT, was isolated from marine sponge Diacarnus erythraeanus. Cells of strain MI-GT are Gram-stain-negative, aerobic, and rod or coccoid-ovoid in shape. MI-GT is able to grow at 10-40 °C (optimum, 28 °C), with 1.0-8.0% (w/v) NaCl (optimum, 4.0%), and at pH 5.5-9.0 (optimum, pH 8.0). The 16S rRNA gene sequence of strain MI-GT shows 98.35, 97.32 and 97.25% similarity to those of Microbulbifer variabilis Ni-2088T, Microbulbifer maritimus TF-17T and Microbulbifer echini AM134T, respectively. Phylogenetic analysis also exhibits that strain MI-GT falls within a clade comprising members of the genus Microbulbifer (class Gammaproteobacteria). The genome size of strain MI-GT is 4478124 bp with a G+C content of 54.51 mol%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MI-GT and other type strains are 71.61-76.44% (ANIb), 83.27-84.36% (ANIm) and 13.4-18.7% (dDDH), respectively. These values are significantly lower than the recommended threshold values for bacterial species delineation. Percentage of conserved proteins and average amino acid identity values among the genomes of strain MI-GT and other closely related species are 52.04-59.13% and 67.47-77.21%, respectively. The major cellular fatty acids of MI-GT are composed of summed feature 8 (C18 : 1 ω7c or C18 : 1 ω6c), iso-C11 : 0 3-OH, iso-C15 : 0, C16 : 0, and summed feature 9 (C17 : 1 iso ω9c or C16 : 0 10-methyl). The polar lipids of MI-GT mainly consist of phosphatidylethanolamine, phosphatidylglycerol, aminolipid, and two glycolipids. The major respiratory quinone is Q-8. Based on differential phenotypic and phylogenetic data, strain MI-GT is considered to represent a novel species of genus Microbulbifer, for which the name Microbulbifer spongiae sp. nov. is proposed. The type strain is MI-GT (=MCCC 1K07826T=KCTC 8081T).

从海洋海绵 Diacarnus erythraeanus 中分离出的 Microbulbifer spongiae sp.
从海洋海绵 Diacarnus erythraeanus 中分离出一种新型细菌,命名为 MI-GT。菌株 MI-GT 的细胞为革兰氏阴性,需氧,呈棒状或茧卵形。MI-GT 能在 10-40 °C(最适 28 °C)、1.0-8.0%(w/v)NaCl(最适 4.0%)和 pH 值 5.5-9.0 (最适 pH 值 8.0)条件下生长。菌株 MI-GT 的 16S rRNA 基因序列与 Microbulbifer variabilis Ni-2088T、Microbulbifer maritimus TF-17T 和 Microbulbifer echini AM134T 的相似度分别为 98.35%、97.32%和 97.25%。系统进化分析还表明,MI-GT 菌株属于微球藻属(伽马蛋白菌类)的一个支系。菌株 MI-GT 的基因组大小为 4478124 bp,G+C 含量为 54.51 mol%。MI-GT 菌株与其他类型菌株的平均核苷酸同一性(ANI)和数字 DNA-DNA 杂交(dDDH)值分别为 71.61%-76.44%(ANIb)、83.27%-84.36%(ANIm)和 13.4%-18.7%(dDDH)。这些数值明显低于细菌物种划分的推荐阈值。MI-GT 菌株和其他近缘物种基因组中保守蛋白质的百分比和平均氨基酸相同值分别为 52.04%-59.13%和 67.47%-77.21%。MI-GT 的主要细胞脂肪酸由总特征 8(C18 : 1 ω7c 或 C18 : 1 ω6c)、异 C11 : 0 3-OH、异 C15 : 0、C16 : 0 和总特征 9(C17 : 1 异 ω9c 或 C16 : 0 10-甲基)组成。MI-GT 的极性脂质主要由磷脂酰乙醇胺、磷脂酰甘油、氨基脂质和两种糖脂组成。主要的呼吸醌是 Q-8。根据不同的表型和系统发育数据,MI-GT 菌株被认为代表了微囊藻属的一个新种,因此被命名为微囊藻海绵属新种(Microbulbifer spongiae sp.模式菌株为 MI-GT(=MCCC 1K07826T=KCTC 8081T)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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