Online Isotope Analysis of Sulfur in Proteins via Capillary Electrophoresis Coupled With Multicollector ICP-MS (CE/MC-ICP-MS): A Proof of Concept Study.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2024-09-30 DOI:10.1002/elps.202400128

Dariya Tukhmetova, Nicole Langhammer, Jochen Vogl, Björn Meermann

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引用次数: 0

Abstract

Isotope ratio analysis of sulfur in biological samples using inductively coupled plasma-mass spectrometry (ICP-MS) has gained significant interest for applications in quantitative proteomics. Advancements like coupling separation techniques with multicollector ICP-MS (MC-ICP-MS) enhance the throughput of species-specific sulfur isotope ratio measurements, fostering new avenues for studying sulfur metabolism in complex biological matrices. This proof-of-concept study investigates the feasibility of online CE/MC-ICP-MS for directly analyzing sulfur isotope ratios in proteins (albumin). Leveraging our previous work on the applicability of CE/ICP-MS for quantifying sulfur-containing biological molecules, we explore its potential for sulfur isotope analysis. Our results demonstrate that direct analysis of sulfur isotopes in albumin protein using online capillary electrophoresis MC-ICP-MS (CE/MC-ICP-MS) eliminates the need for laborious pretreatment steps, while yielding isotope ratios comparable to the reference values. Although initial precision can be improved through further system optimization and protein injection techniques, this approach paves the way for future analysis of mixtures of various biological compounds in, for example, clinical diagnosis studies.

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通过毛细管电泳与多收集器 ICP-MS (CE/MC-ICP-MS) 联机分析蛋白质中硫的同位素：概念验证研究。

利用电感耦合等离子体质谱法（ICP-MS）对生物样本中的硫进行同位素比值分析，在定量蛋白质组学中的应用受到了极大关注。将分离技术与多收集器 ICP-MS （MC-ICP-MS）耦合等先进技术提高了物种特异性硫同位素比测量的通量，为研究复杂生物基质中的硫代谢开辟了新途径。这项概念验证研究调查了在线 CE/MC-ICP-MS 直接分析蛋白质（白蛋白）中硫同位素比值的可行性。利用 CE/ICP-MS 在量化含硫生物分子方面的应用，我们探索了其在硫同位素分析方面的潜力。我们的研究结果表明，利用在线毛细管电泳 MC-ICP-MS （CE/MC-ICP-MS）直接分析白蛋白中的硫同位素无需费力的前处理步骤，同时还能得到与参考值相当的同位素比值。虽然最初的精确度还可以通过进一步的系统优化和蛋白质注射技术来提高，但这种方法为今后分析各种生物化合物混合物（例如临床诊断研究）铺平了道路。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.