Exploring the HPLC Profiling and Antioxidant Potency in Methanolic Extracts of Curcuma longa L. Rhizomes.

IF 1.7 Q3 PHARMACOLOGY & PHARMACY
Drug Research Pub Date : 2024-11-01 Epub Date: 2024-09-26 DOI:10.1055/a-2413-3740
Babita Shukla, Poonam Kushwaha
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引用次数: 0

Abstract

In this exploration of Curcuma longa Linn., commonly known as turmeric, renowned for its therapeutic history, our study focuses on the bioactive compound curcumin. We established and validated an HPLC method for precise curcumin quantification, complemented by an examination of total phenolic and flavonoid content, as well as antioxidant capabilities in a methanolic extract from C. longa rhizomes obtained through a methanol-based Soxhlet extraction. The HPLC analysis utilized a C18 column with a mobile phase of 70:30 V/V Acetonitrile (ACN) to Water (with 1% Glacial Acetic Acid) under an isocratic elution at 1 mL/min, detection at 420 nm, and a reference standard. The method exhibited notable accuracy, precision, and reproducibility. Antioxidant potential, assessed through DPPH, nitric oxide, and hydroxyl radical scavenging assays, unveiled significant total phenol and total flavonoid quantities, highlighting C. longa's potent antioxidant capacity. The study demonstrated a correlation between antioxidant activities and phenolic and flavonoid concentrations, reinforcing the robustness of our method. In conclusion, this method offers an effective means of quantifying curcumin within C. longa rhizomes and holds promise as a valuable tool for maintaining quality control in the production of this botanical material.

探索莪术根茎甲醇提取物的高效液相色谱分析和抗氧化能力
姜黄俗称 "莪术",因其治疗效果显著而闻名于世,我们的研究重点是生物活性化合物姜黄素。我们建立并验证了一种用于精确定量姜黄素的 HPLC 方法,同时还检测了通过基于甲醇的索氏提取法从姜黄根茎中提取的甲醇提取物中的总酚和类黄酮含量以及抗氧化能力。高效液相色谱分析采用 C18 色谱柱,流动相为 70:30 V/V 乙腈(ACN)-水(含 1% 冰醋酸),等度洗脱,流速为 1 mL/min,检测波长为 420 nm,并使用了标准物质。该方法具有显著的准确度、精密度和重现性。通过 DPPH、一氧化氮和羟基自由基清除测定评估了抗氧化潜力,结果显示总酚和总黄酮的含量很高,突出了龙牙草强大的抗氧化能力。研究表明,抗氧化活性与酚类和类黄酮的浓度之间存在相关性,这增强了我们方法的稳健性。总之,这种方法为量化龙牙草根茎中的姜黄素提供了一种有效的手段,有望成为保持这种植物材料生产质量控制的重要工具。
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来源期刊
Drug Research
Drug Research PHARMACOLOGY & PHARMACY-
CiteScore
3.50
自引率
0.00%
发文量
67
期刊介绍: Drug Research (formerly Arzneimittelforschung) is an international peer-reviewed journal with expedited processing times presenting the very latest research results related to novel and established drug molecules and the evaluation of new drug development. A key focus of the publication is translational medicine and the application of biological discoveries in the development of drugs for use in the clinical environment. Articles and experimental data from across the field of drug research address not only the issue of drug discovery, but also the mathematical and statistical methods for evaluating results from industrial investigations and clinical trials. Publishing twelve times a year, Drug Research includes original research articles as well as reviews, commentaries and short communications in the following areas: analytics applied to clinical trials chemistry and biochemistry clinical and experimental pharmacology drug interactions efficacy testing pharmacodynamics pharmacokinetics teratology toxicology.
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