Characterization and Promising in vitro Antiherpetic Effect of Galactomannan from Delonix regia Seeds.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-09-25 DOI:10.1007/s00284-024-03903-6

Jonatas Fernando Cavalini de Moraes, Daniele Zendrini Rechenchoski, André Luiz Dyna, Arcelina Pacheco Cunha, Nágila Maria Pontes Silva Ricardo, Silvana Silveira de Farias, Selene Maia de Morais, Lucy Megumi Yamauchi, Ligia Carla Faccin-Galhardi

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Abstract

Herpes simplex virus (HSV) infections can occur throughout life, thereby allowing transmission to new hosts, with an impact on public health. Acyclovir remains the treatment of choice for these infections; however, an increase in resistant strains in recent years has been observed. In this study, the activity of a native Delonix regia galactomannan (NDr) against HSV-1 was investigated in vitro. NDr was characterized using infrared spectroscopy and NMR. Evaluation of cytotoxicity and the antiviral effect was determined, respectively, by MTT and plaque reduction assays. The NDr concentrations that inhibited cell viability (CC₅₀) and viral infection (IC₅₀) by 50% were above 2000 and 64 μg/mL, respectively. Thus, the polysaccharide showed a high selectivity index (> 31.25). When NDr was added at different stages of HSV-1 replication, a strong inhibitory effect was found by direct interaction with the virus (71-67%, virucidal effect) or previously with the cell, 6 h before infection (99.8-68.4%, prophylactic effect) at concentrations from 200 to 50 μg/mL. NDr showed similar effects in prophylactic 1 h (52%) and adsorption inhibition (55%) assays at 200 μg/mL. A reduction in the antiherpetic effect was observed after infection. These results suggest that NDr is effective in the early stages of HSV-1 infection and is a promising agent for controlling herpetic infections.

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德洛尼西娅种子中的半乳甘露聚糖的特征和体外抗带状疱疹的良好效果

单纯疱疹病毒（HSV）感染可发生在人的一生中，从而传播给新的宿主，对公共卫生造成影响。阿昔洛韦仍然是治疗此类感染的首选药物，但近年来发现耐药株有所增加。本研究在体外研究了一种原生德洛尼西娅半乳甘露聚糖（NDr）对 HSV-1 的活性。使用红外光谱和核磁共振对 NDr 进行了表征。细胞毒性和抗病毒效果的评估分别通过 MTT 和斑块缩小试验进行。抑制细胞活力（CC50）和病毒感染（IC50）50%的 NDr 浓度分别超过 2000 和 64 μg/mL。因此，多糖显示出较高的选择性指数（> 31.25）。当在 HSV-1 复制的不同阶段添加 NDr 时，通过与病毒的直接作用（71-67%，杀病毒效果）或之前与细胞的作用（感染前 6 小时，99.8-68.4%，预防效果），NDr 在 200 至 50 μg/mL 的浓度范围内具有很强的抑制效果。在 200 微克/毫升浓度下，NDr 在 1 小时预防效果（52%）和吸附抑制效果（55%）试验中显示出相似的效果。感染后，抗疱疹效果有所下降。这些结果表明，NDr 在 HSV-1 感染的早期阶段有效，是一种很有前景的控制疱疹感染的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464