Experimental Model of Cerebral Arterial Air Embolism in Conscious Rats.

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-01 Epub Date: 2024-10-01 DOI:10.1007/s10517-024-06246-1

V A Palikov, A N Murashev, R R Amirov, A M Ismailova, V A Kazakov, E S Sadovnikova, Yu A Palikova, I D Rubinshteyn, S O Afanasyeva, E S Mikhaylov, S G Semushina, I A Dyachenko, A T Logunov, N B Pavlov

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Abstract

In this work, an optimal air supply mode was selected to create a model of cerebral arterial air embolism (CAAE) on conscious male Sprague-Dawley rats (n=49). The efficacy of the selected model (administration of 100 μl/kg of air at a rate of 10 μl/min with an infusion pump) was determined by changes in serum biochemical parameters (cholesterol, alkaline phosphatase, inorganic phosphates, AST, and triglycerides), impaired motor functions in the Rotarod test, and visual assessment of the ischemic foci (staining of frontal sections with 1% triphenyltetrazolium chloride solution) at different terms after AAE. The model of AAE created by us confirmed impairment of coordination and motor function in conscious animals and reproduced the lethal consequences of this condition. The obtained results can serve as the basis for drug testing and the development of new approaches to the treatment of ischemic stroke.

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清醒大鼠脑动脉空气栓塞实验模型

这项研究选择了一种最佳供气模式，在意识清醒的雄性 Sprague-Dawley 大鼠（49 只）身上建立了脑动脉空气栓塞（CAAE）模型。所选模型的疗效（用输液泵以 10 μl/min 的速度输入 100 μl/kg 的空气）是通过 AAE 后不同时期大鼠血清生化指标（胆固醇、碱性磷酸酶、无机磷酸盐、谷草转氨酶和甘油三酯）的变化、旋转木马试验中运动功能的受损以及缺血灶的目测评估（用 1%三苯基氯化四氮唑溶液对额叶切片染色）来确定的。我们创建的 AAE 模型证实了有意识动物的协调和运动功能受损，并再现了这种情况的致命后果。获得的结果可作为药物测试和开发治疗缺血性中风新方法的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.