A time-saving one-step polyacrylamide gel with a colored stacking gel for SDS-PAGE and western blotting

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2024-09-26 DOI:10.1016/j.ab.2024.115680

引用次数: 0

Abstract

A time-saving, one-step polyacrylamide gel preparation method enabling simultaneous preparation of separating and stacking gels was previously reported, but the boundary between the separating and stacking gels was often not well defined. As such, determining whether the gel preparation failed is difficult before SDS-PAGE is carried out. To address this issue, a one-step polyacrylamide gel preparation method was developed in which the stacking gel is colored to allow better visualization of the border between the stacking and separating gels. This new one-step method saves time and achieves comparable performance for SDS-PAGE and western blotting to that obtained with conventional gels.

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一步式聚丙烯酰胺凝胶，带彩色叠层凝胶，用于 SDS-PAGE 和 Western 印迹，省时省力。

以前曾报道过一种省时的一步法聚丙烯酰胺凝胶制备方法，可同时制备分离凝胶和堆积凝胶，但分离凝胶和堆积凝胶之间的界限往往界定不清。因此，在进行 SDS-PAGE 分析之前很难确定凝胶制备是否失败。为了解决这个问题，我们开发了一种一步法聚丙烯酰胺凝胶制备方法，该方法对堆积凝胶进行着色，以便更好地观察堆积凝胶和分离凝胶之间的边界。这种新的一步法不仅节省了时间，而且在 SDS-PAGE 和 Western 印迹方面的性能与传统凝胶不相上下。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.