Utilization of Assisted Reproductive Technologies in Breeding Auliekol Cattle: A Comparative Study.

IF 3.2 3区生物学 Q1 BIOLOGY

Life-Basel Pub Date : 2024-09-15 DOI:10.3390/life14091167

Altyn Kulpiisova, Kairly Yessengaliyev, Gulsara Kassimova, Ainat Kozhakhmetova, Bakytkanym Kadraliyeva, Abeldinov Rustem, Alma Temirzhanova, Nadezhda Burambayeva, Salbak Chylbak-Ool, Elena Pakhomova, Nurzhan Abekeshev, Gulnara Baikadamova, Zhomart Kemeshev, Alexandra Tegza, Arman Issimov, Peter White

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Abstract

This study evaluates the utilization of in vitro embryo production (IVEP) technology for the conservation and breeding of the Auliekol cattle breed, a primary beef breed in Kazakhstan facing population decline due to the cessation of breeding programs and the incursion of transboundary diseases. We assessed the effect of consecutive ovum pick-up (OPU) procedures on oocyte yield and embryo production in Auliekol and Aberdeen Angus cows. A total of 2232 and 3659 oocytes were aspirated from Auliekol and Aberdeen Angus donors, respectively, with significantly higher yields and embryo production observed in Aberdeen Angus cows. The application of a meiotic block using Butyrolactone I (BLI) and subsequent in vitro fertilization (IVF) protocols was employed, with embryo development monitored up to the morula/blastocyst stage. Results indicated that Auliekol cows exhibited lower oocyte recovery, cleavage, and blastocyst rates compared to Aberdeen Angus cows, likely due to genetic characteristics. Despite the challenges, IVEP presents a valuable tool for the preservation and future propagation of the Auliekol breed, highlighting the need for further research to enhance reproductive outcomes and conservation strategies.

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辅助生殖技术在奥利科尔牛育种中的应用：比较研究

本研究评估了体外胚胎生产（IVEP）技术在保护和培育 Auliekol 牛品种方面的应用情况，该品种是哈萨克斯坦的一个主要肉牛品种，由于育种计划的停止和跨境疾病的入侵，该品种的数量正在下降。我们评估了连续取卵（OPU）程序对奥利科尔牛和阿伯丁安格斯牛卵母细胞产量和胚胎产量的影响。从 Auliekol 和 Aberdeen Angus 供体中分别抽取了 2232 和 3659 个卵母细胞，观察到 Aberdeen Angus 奶牛的卵母细胞产量和胚胎产量明显更高。使用丁内酯 I（BLI）进行减数分裂阻断，随后采用体外受精（IVF）方案，监测胚胎发育至小白鼠/囊胚阶段。结果表明，与阿伯丁安格斯奶牛相比，奥利科奶牛的卵母细胞回收率、卵裂率和囊胚率都较低，这可能是遗传特性造成的。尽管面临诸多挑战，IVEP 仍为奥利科尔品种的保存和未来繁殖提供了一个宝贵的工具，突出了进一步研究以提高繁殖结果和保护策略的必要性。

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来源期刊

Life-Basel Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology

CiteScore

4.30

自引率

6.20%

发文量

1798

审稿时长

11 weeks

期刊介绍： Life (ISSN 2075-1729) is an international, peer-reviewed open access journal of scientific studies related to fundamental themes in Life Sciences, especially those concerned with the origins of life and evolution of biosystems. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers.