Detection and prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks in eastern Poland.

IF 1.3 4区医学 Q4 ENVIRONMENTAL SCIENCES

Annals of Agricultural and Environmental Medicine Pub Date : 2024-09-25 Epub Date: 2024-09-18 DOI:10.26444/aaem/192238

Anna Sawczyn-Domańska, Angelina Wójcik-Fatla

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引用次数: 0

Abstract

Introduction: Anaplasma phagocytophilum are tick-borne bacteria affecting both human and animal health. The aim of the study was to examine the prevalence of A. phagocytophilum in questing I. ricinus ticks collected in Lublin Province, eastern Poland.

Material and methods: Ticks were collected by the flagging method. Total DNA from ticks was extracted by boiling in ammonium hydroxide. Detection of A. phagocytophilum was performed by amplifying a fragment of the 16S rDNA gene.

Results: Overall, 626 I. ricinus ticks were tested for the presence of A. phagocytophilum DNA. The prevalence of the pathogenic bacteria was 1.28%. The occurrence of A. phagocytophilum among adults was 1.8%, whereas none of the collected I. ricinus nymphs were infected.

Conclusions: The study revealed the presence of A. phagocytophilum in I. ricinus in eastern Poland, which constitutes a potential health risk for residents, tourists, forestry, and agricultural workers.

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波兰东部蓖麻蜱中噬细胞嗜血杆菌的检测和流行情况。

导言：噬细胞阿纳疟原虫是一种蜱媒细菌，会影响人类和动物的健康。本研究的目的是检测在波兰东部卢布林省采集的蓖麻蜱中噬菌体的流行情况：材料和方法：蜱虫采用旗标法采集。用氢氧化铵煮沸提取蜱虫的总 DNA。通过扩增 16S rDNA 基因片段来检测噬细胞甲虫：结果：共检测了 626 只 I. ricinus 蜱虫体内是否含有噬细胞噬菌体 DNA。病原菌的感染率为 1.28%。成虫中噬菌体的感染率为 1.8%，而采集到的蓖麻若虫中没有一只受到感染：这项研究揭示了波兰东部的蓖麻若虫中存在噬菌体，这对居民、游客、林业和农业工人的健康构成了潜在风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Agricultural and Environmental Medicine 医学-公共卫生、环境卫生与职业卫生

CiteScore

3.00

自引率

5.90%

发文量

审稿时长

4-8 weeks

期刊介绍： All papers within the scope indicated by the following sections of the journal may be submitted: Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases). Health effects of chemical pollutants in agricultural areas , including occupational and non-occupational effects of agricultural chemicals (pesticides, fertilizers) and effects of industrial disposal (heavy metals, sulphur, etc.) contaminating the atmosphere, soil and water. Exposure to physical hazards associated with the use of machinery in agriculture and forestry: noise, vibration, dust. Prevention of occupational diseases in agriculture, forestry, food industry and wood industry. Work-related accidents and injuries in agriculture, forestry, food industry and wood industry: incidence, causes, social aspects and prevention. State of the health of rural communities depending on various factors: social factors, accessibility of medical care, etc.