Distribution and morphology of serotonin transporter-immunoreactive type I cells in the rat carotid body.

4区 医学 Q2 Agricultural and Biological Sciences
Anatomical Record Pub Date : 2024-09-27 DOI:10.1002/ar.25575
Takuya Yokoyama, Sayed Sharif Abdali, Nobuaki Nakamuta, Yoshio Yamamoto
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Abstract

The present study reexamined the immunolocalization of membranous serotonin transporter (SERT) in the rat carotid body, and demonstrated SERT-immunoreactive cells of unreported morphology. SERT was immunohistochemically localized in a very small population of cell clusters or single type I cells (2.8%) immunoreactive for synaptophysin, the marker of these cells. Intense SERT immunoreactivity outlined the perinuclear cytoplasm and multiple cytoplasmic processes of type I cells. Of SERT-immunoreactive type I cells, 14.6% and 32.6% were immunoreactive for tyrosine hydroxylase (TH) and dopamine beta-hydroxylase, respectively, while 75.9% were immunoreactive for serotonin (5-HT). 5-HT-immunoreactive products were localized in cell bodies rather than cytoplasmic processes. SERT-immunoreactive type I cells were composed of an oval cell body with multiple threads and spherical or elongated cytoplasmic processes. Clusters or single SERT-immunoreactive type I cells were localized between or attached to other TH-immunoreactive type I cells by cell bodies or variform cytoplasmic processes. SERT-immunoreactive type I cells mainly contained bassoon-immunoreactive products in their cell bodies rather than their variform cytoplasmic processes. These results demonstrated the characteristic morphology of SERT-immunoreactive type I cells, which extend multiple cytoplasmic processes with variform terminal parts. Their morphology might be suitable for uptake of 5-HT to control the serotonergic modulation in the carotid body.

大鼠颈动脉体中血清素转运体免疫反应 I 型细胞的分布和形态。
本研究对大鼠颈动脉体中膜5-羟色胺转运体(SERT)的免疫定位进行了重新研究,并发现了具有未报道形态的SERT免疫反应细胞。SERT通过免疫组织化学定位在极少量的细胞簇或单个I型细胞(2.8%)中,这些细胞对突触素具有免疫反应,突触素是这些细胞的标记。强烈的SERT免疫反应勾勒出I型细胞的核周细胞质和多个细胞质过程。在SERT免疫反应的I型细胞中,14.6%和32.6%分别对酪氨酸羟化酶(TH)和多巴胺β-羟化酶有免疫反应,而75.9%对5-羟色胺(5-HT)有免疫反应。5-HT免疫反应产物定位于细胞体而非胞质过程。SERT免疫反应的I型细胞由椭圆形细胞体和多条线状、球形或拉长的细胞质突起组成。成群的或单个的SERT免疫反应I型细胞位于其他TH免疫反应I型细胞之间,或通过细胞体或变异的细胞质突起附着在其他TH免疫反应I型细胞上。SERT免疫反应I型细胞的细胞体中主要含有巴松免疫反应产物,而不是其变异型胞质突起。这些结果表明了SERT免疫反应I型细胞的特征性形态,即延伸出多个胞质过程,末端部分呈变异形。它们的形态可能适合摄取 5-HT 以控制颈动脉体的血清素能调节。
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来源期刊
Anatomical Record
Anatomical Record Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics
CiteScore
4.30
自引率
0.00%
发文量
0
期刊介绍: The Anatomical Record
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