Detection and Genetic Characterization of Border Disease Virus (BDV) Isolated from a Persistently Infected Sheep in a Migratory Flock from Rajasthan State, Northwestern India.

IF 3.8 3区医学 Q2 VIROLOGY

Viruses-Basel Pub Date : 2024-08-30 DOI:10.3390/v16091390

Semmannan Kalaiyarasu, Katherukamem Rajukumar, Niranjan Mishra, Shashi Bhusan Sudhakar, Vijendra Pal Singh

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引用次数: 0

Abstract

Border disease virus (BDV) causes significant economic losses in sheep farming worldwide. In India, BDV has not yet been studied in sheep migrating for summer pasturing. This study aimed to determine the extent of BDV infection in migratory sheep and provide genetic characteristics of BDV. Blood and serum samples from 90 lambs of a migratory sheep flock (600) in Central India were collected and subjected to molecular detection, phylogenetic analysis and virus neutralization test (VNT). We detected BDV in two lambs through real-time RT-PCR, while 64.4% (58/90) of in-contact lambs had BDV neutralizing antibodies. One apparently healthy lamb was found to be persistently infected with BDV. Phylogenetic analysis of 5'-UTR and N^pro genes and the concatenated datasets typed the BDV isolate from PI sheep as BDV-3 genotype. However, it showed a closer relationship with BDV-3 strains from China than the previously reported Indian BDV-3 strains. This is the first report on the detection of BDV persistently infected migratory sheep in India. Additionally, we provided evidence of genetic variability among BDV-3 strains in India. The findings improve our understanding of epidemiology and genetic characteristics of BDV in India and highlight the potential risks associated with the traditional practice of sheep migration for summer pasturing.

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从印度西北部拉贾斯坦邦一个迁徙羊群中持续感染的绵羊身上分离出的边境病病毒 (BDV) 的检测和遗传特征。

边境病病毒（BDV）给全世界的养羊业造成了巨大的经济损失。在印度，尚未对夏季放牧的迁徙绵羊感染 BDV 的情况进行研究。本研究旨在确定迁徙绵羊感染 BDV 的程度，并提供 BDV 的遗传特征。我们采集了印度中部一个迁徙羊群（600 只）中 90 只羔羊的血液和血清样本，并对其进行了分子检测、系统发育分析和病毒中和试验（VNT）。我们通过实时 RT-PCR 检测出两只羔羊体内存在 BDV，64.4%（58/90）的接触羔羊体内存在 BDV 中和抗体。发现一只表面健康的羔羊持续感染了 BDV。5'-UTR和Npro基因的系统发育分析以及合并数据集将郫县绵羊的BDV分离株分型为BDV-3基因型。然而，与之前报道的印度 BDV-3 株系相比，它与中国的 BDV-3 株系关系更为密切。这是首次报告在印度发现持续感染 BDV 的迁徙绵羊。此外，我们还提供了印度 BDV-3 株系间遗传变异的证据。这些发现增进了我们对印度 BDV 流行病学和遗传特征的了解，并强调了绵羊夏季放牧迁移这一传统习俗的潜在风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Viruses-Basel VIROLOGY-

CiteScore

7.30

自引率

12.80%

发文量

2445

审稿时长

1 months

期刊介绍： Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.