Evolutionary origin and population diversity of a cryptic hybrid pathogen

IF 14.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2024-09-28 DOI:10.1038/s41467-024-52639-1

Jacob L. Steenwyk, Sonja Knowles, Rafael W. Bastos, Charu Balamurugan, David Rinker, Matthew E. Mead, Christopher D. Roberts, Huzefa A. Raja, Yuanning Li, Ana Cristina Colabardini, Patrícia Alves de Castro, Thaila Fernanda dos Reis, Adiyantara Gumilang, María Almagro-Molto, Alexandre Alanio, Dea Garcia-Hermoso, Endrews Delbaje, Laís Pontes, Camila Figueiredo Pinzan, Angélica Zaninelli Schreiber, David Canóvas, Rafael Sanchez Luperini, Katrien Lagrou, Egídio Torrado, Fernando Rodrigues, Nicholas H. Oberlies, Xiaofan Zhou, Gustavo H. Goldman, Antonis Rokas

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Abstract

Cryptic fungal pathogens pose disease management challenges due to their morphological resemblance to known pathogens. Here, we investigated the genomes and phenotypes of 53 globally distributed isolates of Aspergillus section Nidulantes fungi and found 30 clinical isolates—including four isolated from COVID-19 patients—were A. latus, a cryptic pathogen that originated via allodiploid hybridization. Notably, all A. latus isolates were misidentified. A. latus hybrids likely originated via a single hybridization event during the Miocene and harbor substantial genetic diversity. Transcriptome profiling of a clinical isolate revealed that both parental subgenomes are actively expressed and respond to environmental stimuli. Characterizing infection-relevant traits—such as drug resistance and growth under oxidative stress—revealed distinct phenotypic profiles among A. latus hybrids compared to parental and closely related species. Moreover, we identified four features that could aid A. latus taxonomic identification. Together, these findings deepen our understanding of the origin of cryptic pathogens.

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一种隐性杂交病原体的进化起源和种群多样性

隐性真菌病原体与已知病原体形态相似，给疾病管理带来了挑战。在这里，我们研究了 53 个全球分布的曲霉科尼杜兰真菌分离物的基因组和表型，发现 30 个临床分离物--包括从 COVID-19 患者中分离出的 4 个--是通过异源二倍体杂交产生的隐性病原体 A. latus。值得注意的是，所有分离出的 A. latus 都被误认。A. latus杂交种很可能起源于中新世时期的一次杂交，并蕴藏着巨大的遗传多样性。对一个临床分离株的转录组分析表明，两个亲本亚基因组都能积极表达并对环境刺激做出反应。对感染相关性状（如抗药性和氧化应激下的生长）的特征分析显示，与亲本和近缘物种相比，拉特氏菌杂交种的表型特征截然不同。此外，我们还发现了四种有助于花叶病毒分类鉴定的特征。这些发现共同加深了我们对隐性病原体起源的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.