Description of three new Pseudomonas species isolated from aquarium fish: Pseudomonas auratipiscis sp. nov., Pseudomonas carassii sp. nov. and Pseudomonas ulcerans sp. nov

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology Pub Date : 2024-09-19 DOI:10.1016/j.syapm.2024.126552

Muhammed Duman , Jorge Lalucat , Izzet Burcin Saticioglu , Magdalena Mulet , Margarita Gomila , Soner Altun , Nihed Ajmi , Elena García-Valdés

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引用次数: 0

Abstract

Pseudomonas species constitute a significant group of pathogens in aquarium fish and frequently cause haemorrhagic septicaemia. This study conducted a taxonomic characterization of Pseudomonas isolates from aquarium fish exhibiting deep ulceration and general disease signs. A polyphasic approach was employed to ascertain the taxonomic affiliation of the strains. The overall genome relatedness indices of digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) between the strains and the other members of the genus Pseudomonas were found to be below the established thresholds of 70 and 95–96%, respectively. Whole-genome based phylogenetic analysis revealed that strains 119P^T and 120P were closely related to P. arcuscaelestis. Strain 137P^T was related to P. peradeniyensis, while strains 147P^T and 148P were closely related to P. japonica. The morphological, physiological, and biochemical characteristics of the strains and the genome relatedness indices of dDDH and ANI below the established thresholds confirmed the classification of the strains as three novel species. Genome analyses of the strains were also conducted to determine their biosynthesis-related gene clusters, virulence features and ecological distribution patterns. Based on polyphasic characterization, the strains 119P^T, 120P, 137P^T, 147P^T, and 148P are novel species within the genus Pseudomonas, for which the following names are proposed: Pseudomonas auratipiscis sp. nov., with the strain 119P^T as the type strain (=DSM 117162 ^T, =LMG 33381^T); Pseudomonas carassii sp. nov., with the strain 137P^T as the type strain (=DSM 117060^T, =LMG 33378^T); and Pseudomonas ulcerans sp. nov. 147P^T, as the type strain (=DSM 117163^T, =LMG 33377^T).

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描述从水族鱼类中分离出的三种新假单胞菌：Pseudomonas auratipiscis sp.

假单胞菌是水族鱼类的重要病原体之一，经常引起出血性败血症。本研究对从表现出深度溃疡和一般疾病症状的水族鱼中分离出的假单胞菌进行了分类鉴定。采用多相法来确定菌株的分类归属。发现这些菌株与假单胞菌属其他成员之间的数字 DNA-DNA 杂交（dDDH）和平均核苷酸同一性（ANI）的整体基因组亲缘关系指数分别低于 70% 和 95-96% 的既定阈值。基于全基因组的系统发育分析表明，菌株 119PT 和 120P 与 P. arcuscaelestis 关系密切。菌株 137PT 与 P. peradeniyensis 关系密切，而菌株 147PT 和 148P 与 P. japonica 关系密切。这些菌株的形态、生理和生化特征以及 dDDH 和 ANI 的基因组亲缘关系指数低于既定阈值，证实了这些菌株被划分为三个新物种。此外，还对菌株进行了基因组分析，以确定其生物合成相关基因簇、毒力特征和生态分布模式。根据多相特征，119PT、120P、137PT、147PT 和 148P 株系是假单胞菌属中的新物种，现将其命名如下：以 119PT 株为模式菌株（=DSM 117162 T，=LMG 33381T）；以 137PT 株为模式菌株（=DSM 117060T，=LMG 33378T）；以 147PT 株为模式菌株（=DSM 117163T，=LMG 33377T）。

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来源期刊

Systematic and applied microbiology 生物-生物工程与应用微生物

CiteScore

7.50

自引率

5.90%

发文量

审稿时长

22 days

期刊介绍： Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: