{"title":"The protective effect of two highly branched polysaccharides from corn silk fermented by Lactobacillus plantarum against acute liver injury","authors":"","doi":"10.1016/j.carbpol.2024.122799","DOIUrl":null,"url":null,"abstract":"<div><div>To valorize the natural resource of corn silk as hepatoprotective food supplement, two fractions of polysaccharides (F-CSP-W/S) have been separated and purified from corn silk fermented by <em>Lactobacillus plantarum</em>. F-CSP-W was mainly composed of Glc, Gal, Ara, Xyl, Man (66.62: 11.83: 11.59: 7.89:1.48), traces of GlcN and GlcA; whereas F-CSP-S was mainly composed of Glc, Xyl, Gal, Ara, GlcA, GalA, Rha, Man (39.80: 18.26: 17.04: 11.87: 3.33: 3.18: 3.04: 2.77) and traces of GlcN. The detailed chemical structure analysis showed F-CSP-W/S were highly branched polysaccharides, which possessed the backbone of α-1 → 4-Glc<em>p</em> branched at α-1 → 3-Gal<em>p,</em> with the solvated domains of arabinoxylan mainly consisted of β-1 → 4-Xyl, α-Ara and 4-O-Me-α-t-Glcp UA as side chains. F-CSP-S had a higher branch degree with pectin like side chain that contained partial methyl-esterificated α-1 → 4-GalUA and 1 → 2-Rha<em>p</em> covalently bound to arabinoxylan. Meanwhile, F-CSP-S displayed potent anti-oxidant activity <em>in vitro</em>. Both F-CSP-W and F-CSP-S ameliorated CCl<sub>4</sub>-induced mouse acute liver injury through directly hepatoprotective effect as verified by the organ index, liver function, serum enzymatic activities and histological changes of liver.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":null,"pages":null},"PeriodicalIF":10.7000,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Polymers","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0144861724010257","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
To valorize the natural resource of corn silk as hepatoprotective food supplement, two fractions of polysaccharides (F-CSP-W/S) have been separated and purified from corn silk fermented by Lactobacillus plantarum. F-CSP-W was mainly composed of Glc, Gal, Ara, Xyl, Man (66.62: 11.83: 11.59: 7.89:1.48), traces of GlcN and GlcA; whereas F-CSP-S was mainly composed of Glc, Xyl, Gal, Ara, GlcA, GalA, Rha, Man (39.80: 18.26: 17.04: 11.87: 3.33: 3.18: 3.04: 2.77) and traces of GlcN. The detailed chemical structure analysis showed F-CSP-W/S were highly branched polysaccharides, which possessed the backbone of α-1 → 4-Glcp branched at α-1 → 3-Galp, with the solvated domains of arabinoxylan mainly consisted of β-1 → 4-Xyl, α-Ara and 4-O-Me-α-t-Glcp UA as side chains. F-CSP-S had a higher branch degree with pectin like side chain that contained partial methyl-esterificated α-1 → 4-GalUA and 1 → 2-Rhap covalently bound to arabinoxylan. Meanwhile, F-CSP-S displayed potent anti-oxidant activity in vitro. Both F-CSP-W and F-CSP-S ameliorated CCl4-induced mouse acute liver injury through directly hepatoprotective effect as verified by the organ index, liver function, serum enzymatic activities and histological changes of liver.
期刊介绍:
Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience.
The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.