Selection of signature peptide biomarkers for the sesame allergens in commercial food based on LC-MS/MS

Abstract

Sesame is a commonly used food ingredient, yet it is one of the eight major allergens. As sesame is often consumed in various processed forms, it is important to establish methods for detecting sesame allergens in processed foods. Liquid chromatography-tandem mass spectrometry (LC-MS/MS), using characteristic peptides as biomarkers, detects multiple allergenic proteins simultaneously with high sensitivity and accuracy. Choosing robust biomarkers is beneficial for developing a specific, universal, and sensitive method. To obtain excellent peptides of sesame allergens, sixteen commercial products were used as test materials. Proteins from these materials were extracted, digested, and analyzed. Peptides were screened based on several criteria, including specificity and amino acid composition. Only peptides showing process robustness were retained. Ultimately, nine peptides were selected as the best biomarkers. Based on the above peptides, it is possible to achieve precise and high-sensitivity detection of sesame allergens in processed products.